GPI-1046 stimulates chicken dorsal root ganglion neurite outgrowth in the presence of nerve growth factor at low concentration in vitro.

Abstract

The purpose of this investigation was to re-evaluate the neurotrophic effect of GPI-1046 on neurite outgrowth in vitro. GPI-1046 was synthesized and identified with mass spectrometry, nuclear magnetic resonance and elemental analysis. Chicken dorsal root ganglions (DRGs) were removed and divided into three groups: (1) The DRGs were cultured in DMEM containing different concentrations of GPI-1046; (2) The DRGs were cultured in DMEM containing nerve growth factor (NGF) alone at 0.8 and 8 ng/mL, respectively; (3) The DRGs were cultured in DMEM containing both different concentrations of GPI-1046 and NGF at 0.8 ng/mL. The results showed that GPI-1046 alone could not stimulate chicken DRG neurite outgrowth; however, GPI-1046 stimulated DRG neurite outgrowth only in the presence of NGF at low concentration in the culture medium.

• Authors: Hai-Ping Que, Xin Li, Song Li, Shao-Jun Liu
• Journal: Sheng li xue bao : [Acta physiologica Sinica] (Sheng Li Xue Bao) Vol. 59 Issue 6 Pg. 791-5 (Dec 25 2007) ISSN: 0371-0874 [Print] China
• PMID: 18157473 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• GPI 1046
• Pyrrolidines
• Nerve Growth Factor

Topics

• Animals
• Cells, Cultured
• Chickens
• Ganglia, Spinal (drug effects, growth & development)
• Nerve Growth Factor (pharmacology)
• Neurites (drug effects)
• Pyrrolidines (pharmacology)