Preliminary study of the anti-inflammatory activity of hexane extract and fractions from Bursera simaruba (Linneo) Sarg. (Burseraceae) leaves.

Abstract

Bursera simaruba (L.) Sarg. leaves hexane extracts display anti-inflammatory activity on the adjuvant-carrageenan-induced inflammation in rats. In order to isolate and identify the active compounds of the hexane extract, we performed a preliminary phytochemical study and a bioassay-directed fractionation using the carrageenan-induced paw oedema test in mice. From the nine fractions (A-I) obtained, of an initial chromatographic separation, fractions A and E (doses equivalents to 1.50 g dry plant/kg body weight) showed the strongest anti-inflammatory activity comparable to that of the reference drug phenylbutazone (80 mg/kg). The isolation and characterization of 3-methylene-7,11,15-trimethylhexadec-1-ene (neophytadiene) (1), ergost-5-en-3beta-ol (2), 24S-stigmast-5,22E-dien-3beta-ol (3), 24S-stigmast-5-en-3beta-ol (4) and alpha-amyrin (5), from these fractions is reported.

• Authors: M E Carretero, J L López-Pérez, M J Abad, P Bermejo, S Tillet, A Israel, B Noguera-P
• Journal: Journal of ethnopharmacology (J Ethnopharmacol) Vol. 116 Issue 1 Pg. 11-5 (Feb 28 2008) ISSN: 0378-8741 [Print] Ireland
• PMID: 18155865 (Publication Type: Journal Article)

Chemical References

• Anti-Inflammatory Agents
• Plant Extracts
• Tetradecanoylphorbol Acetate

Topics

• Animals
• Anti-Inflammatory Agents (isolation & purification, pharmacology)
• Bursera (chemistry)
• Female
• Mice
• Plant Extracts (analysis, pharmacology)
• Plant Leaves (chemistry)
• Tetradecanoylphorbol Acetate (pharmacology)