Water stress and cell wall polysaccharides in the apical root zone of wheat cultivars varying in drought tolerance.

Abstract

Glycosyl composition and linkage analysis of cell wall polysaccharides were examined in apical root zones excised from water-stressed and unstressed wheat seedlings (Triticum durum Desf.) cv. Capeiti ("drought-tolerant") and cv. Creso ("drought sensitive"). Wall polysaccharides were sequentially solubilized to obtain three fractions: CDTA+Na(2)CO(3) extract, KOH extract and the insoluble residue (alpha-cellulose). A comparison between the two genotypes showed only small variations in the percentages of matrix polysaccharides (CDTA+Na(2)CO(3) plus KOH extract) and of the insoluble residues (alpha-cellulose) in water-stressed and unstressed conditions. Xylosyl, glucosyl and arabinosyl residues represented more than 90 mol% of the matrix polysaccharides. The linkage analysis of matrix polysaccharides showed high levels of xyloglucans (23-39 mol%), and arabinoxylans (38-48 mol%) and a low amount of pectins and (1-->3), (1-->4)-beta-D-glucans. The high level of xyloglucans was supported by the release of the diagnostic disaccharide isoprimeverose after Driselase digestion of KOH-extracted polysaccharides. In the "drought-tolerant" cv. Capeiti the mol% of side chains of rhamnogalacturonan I and II significantly increased in response to water stress, whereas in cv. Creso, this increase did not occur. The results support a role of the pectic side chains during water stress response in a drought-tolerant wheat cultivar.

• Authors: Maria Rosaria Leucci, Marcello Salvatore Lenucci, Gabriella Piro, Giuseppe Dalessandro
• Journal: Journal of plant physiology (J Plant Physiol) Vol. 165 Issue 11 Pg. 1168-80 (Jul 31 2008) ISSN: 1618-1328 [Electronic] Germany
• PMID: 18155804 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Disaccharides
• Fungal Proteins
• Hydroxides
• Plant Extracts
• Polysaccharides
• Potassium Compounds
• isoprimeverose
• Glycoside Hydrolases
• driselase
• potassium hydroxide

Topics

• Adaptation, Physiological
• Carbohydrate Conformation
• Carbohydrate Sequence
• Cell Polarity
• Cell Wall (chemistry)
• Chromatography, Ion Exchange
• Dehydration
• Disaccharides (metabolism)
• Droughts
• Electrophoresis
• Fungal Proteins (metabolism)
• Glycoside Hydrolases (metabolism)
• Hydrolysis
• Hydroxides
• Molecular Sequence Data
• Plant Extracts (metabolism)
• Plant Roots (cytology, metabolism)
• Polysaccharides (chemistry, metabolism)
• Potassium Compounds
• Seedlings (metabolism)
• Triticum (cytology, metabolism)