Synthesis of an anthrose derivative and production of polyclonal antibodies for the detection of anthrax spores.

Abstract

A straightforward synthesis of a derivative of anthrose, the non-reducing terminal fragment of the antigenic tetrasaccharide from Bacillus anthracis, was achieved starting from d-galactose. This hapten is able to induce a highly specific and sensitive immune response in rabbit when attached to a carrier protein.

• Authors: Sandrine G Y Dhénin, Vincent Moreau, Nathalie Morel, Marie-Claire Nevers, Hervé Volland, Christophe Créminon, Florence Djedaïni-Pilard
• Journal: Carbohydrate research (Carbohydr Res) Vol. 343 Issue 12 Pg. 2101-10 (Aug 11 2008) ISSN: 0008-6215 [Print] Netherlands
• PMID: 18155682 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• 4,6-dideoxy-4-(3-hydroxy-3-methylbutanamido)-2-O-methylglucopyranose
• Amino Sugars
• Antibodies, Bacterial
• Hemocyanins
• Deoxyglucose
• keyhole-limpet hemocyanin

Topics

• Amino Sugars (chemical synthesis)
• Animals
• Antibodies, Bacterial (immunology)
• Bacillus anthracis (immunology)
• Deoxyglucose (analogs & derivatives, chemical synthesis)
• Hemocyanins (immunology)
• Immunoenzyme Techniques (methods)
• Rabbits
• Spores, Bacterial (chemistry, immunology)