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Protective effects of naringenin-7-O-glucoside on doxorubicin-induced apoptosis in H9C2 cells.

Abstract
Doxorubicin, a widely used chemotherapeutic agent, can give rise to severe cardiotoxicity by inducing cardiomyocyte apoptosis. Dracocephalum rupestre Hance, a Chinese traditional herb, has therapeutic potential for cardiovascular diseases. Naringenin-7-O-glucoside is the main active constituent of D. rupestre and there is increasing interest in its therapeutic applications. The aim of this study was to evaluate the effects of naringenin-7-O-glucoside on cardiomyocyte apoptosis induced by doxorubicin. Cell viability was detected by MTT assay. Naringenin-7-O-glucoside (10, 20, and 40 microM) significantly enhanced cardiomyocyte proliferation relative to that of doxorubicin. Furthermore, naringenin-7-O-glucoside increased the protein levels of heme oxygenase-1 (HO-1) and Bcl-2 in cardiomyocytes (as detected by Western blotting) and suppressed the mRNA expression of caspase-3 and caspase-9 (as detected by RT-PCR). These results suggest that naringenin-7-O-glucoside has protective effects against doxorubicin-induced apoptosis, effects which could underlie the use of naringenin-7-O-glucoside therapeutic agent for treating or preventing cardiomyopathy associated with doxorubicin.
AuthorsXiuzhen Han, Dongmei Ren, Peihong Fan, Tao Shen, Hongxiang Lou
JournalEuropean journal of pharmacology (Eur J Pharmacol) Vol. 581 Issue 1-2 Pg. 47-53 (Feb 26 2008) ISSN: 0014-2999 [Print] Netherlands
PMID18154951 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Drugs, Chinese Herbal
  • Flavanones
  • Glucosides
  • Proto-Oncogene Proteins c-bcl-2
  • naringenin-7-O-glucoside
  • Doxorubicin
  • Heme Oxygenase-1
  • Caspase 3
  • Caspase 9
Topics
  • Animals
  • Apoptosis (drug effects)
  • Caspase 3 (genetics)
  • Caspase 9 (genetics)
  • Cells, Cultured
  • Cytoprotection
  • Doxorubicin (toxicity)
  • Drugs, Chinese Herbal (pharmacology)
  • Flavanones (pharmacology)
  • Glucosides (pharmacology)
  • Heme Oxygenase-1 (analysis, genetics)
  • Lamiaceae (chemistry)
  • Myocytes, Cardiac (cytology, drug effects)
  • Proto-Oncogene Proteins c-bcl-2 (analysis, genetics)
  • Rats

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