Soluble mesothelin-related peptide level elevation in mesothelioma serum and pleural effusions.

Abstract

BACKGROUND:

Soluble mesothelin-related peptide (SMRP) is a potential marker for malignant pleural mesothelioma (MPM), which may be useful for screening high-risk asbestos-exposed individuals.

METHODS:

We evaluated SMRP in serum from MPM patients (n = 90), lung cancer patients (n = 170), age and tobacco-matched asbestos-exposed individuals (n = 66), and in MPM pleural effusions (n = 45), benign effusions (n = 30), and non-MPM effusions (n = 20) using the MesoMark enzyme-linked immunosorbent assay kit (Fujirebio Diagnostics, Malvern, PA). Receiver operating characteristic (ROC) curves were used to define true and false positive rates at various cutoffs.

RESULTS:

Mean serum SMRP levels were higher in MPM compared with lung cancer (5.67 +/- 0.82 nM [mean +/- standard error of the mean vs 1.99 +/- 0.43 nM, p < 0.001), and stage I MPM SMRP levels (n = 12; 2.09 +/- 0.41 nM) were significantly higher than those in asbestos-exposed individuals (0.99 +/- 0.09 nM, p = 0.02, respectively). Stage 2 to 4 SMRP serum levels were significantly higher than those for stage 1 MPM. The area under the ROC curve for serum SMRP was 0.81 for differentiating MPM and asbestos-exposed individuals; cutoff = 1.9 nM (sensitivity = 60%, specificity = 89%). The MPM pleural effusion SMRP was significantly higher than benign or other non-MPM pleural effusions (65.57 +/- 11.33 nM vs 27.46 +/- 11.25 nM [p = 0.003] and 18.99 +/- 7.48 nM [p = 0.044], respectively).

CONCLUSIONS:

These data support SMRP as a promising marker for MPM in both serum and pleural effusion fluid, and justify prospective screening studies of SMRP in combination with other markers for screening of asbestos-exposed cohorts.

• Authors: Harvey I Pass, Anil Wali, Naimei Tang, Alla Ivanova, Sergey Ivanov, Michael Harbut, Michele Carbone, Jeffrey Allard
• Journal: The Annals of thoracic surgery (Ann Thorac Surg) Vol. 85 Issue 1 Pg. 265-72; discussion 272 (Jan 2008) ISSN: 1552-6259 [Electronic] Netherlands
• PMID: 18154821 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)

Chemical References

• Biomarkers, Tumor
• GPI-Linked Proteins
• Membrane Glycoproteins
• Mesothelin

Topics

• Adult
• Aged
• Aged, 80 and over
• Analysis of Variance
• Area Under Curve
• Asbestosis (blood, complications, mortality, pathology)
• Biomarkers, Tumor (blood)
• Case-Control Studies
• Female
• GPI-Linked Proteins
• Humans
• Immunohistochemistry
• Male
• Membrane Glycoproteins (blood)
• Mesothelin
• Mesothelioma (blood, complications, mortality, pathology)
• Middle Aged
• Neoplasm Staging
• Pleural Effusion, Malignant (blood, etiology)
• Pleural Neoplasms (blood, complications, mortality, pathology)
• Prognosis
• ROC Curve
• Retrospective Studies
• Risk Assessment
• Sensitivity and Specificity
• Survival Analysis