| Abstract | The effects of KF-14363 on active oxygen production and membrane stabilization were studied. KF-14363 did not affect hypotonic hemolysis (10% and 70%) and did not inhibit lipid peroxide production induced by t-butyl hydroperoxide at concentrations of less than 100 microM. KF-14363 significantly inhibited active oxygen production in peritoneal exudate cells (PEEC) stimulated with arachidonic acid, A23187 and carbon tetrachloride (CCl4) at concentrations over 10 microM, 100 microM and 1 microM, respectively. It tended to inhibit formyl-methionyl-leucyl-phenylalanine-stimulated production of active oxygen in PEEC at concentrations over 10 microM, but there was no significant difference owing to large dispersion. Superoxide dismutase (SOD, 10(4) U/ml) significantly inhibited CCl4-stimulated production of active oxygen in PEEC. KF-14363 inhibited the radical production from CCl4 in the presence of a 9000 x g supernatant fraction of the rat liver which was administered with enzyme induction compounds (S9 mix). SOD (10(4) U/ml) was not effective in this system. In conclusion, KF-14363 inhibited active oxygen production in PEEC induced by various stimulants and also the radical formation from CCl4 in the presence of S9 mix solution. |
| Authors | I Yoshitake, K Kubo
(Affiliation: Pharmaceutical Research Laboratories, Kyowa Hakko Kogyo Co., Ltd., Shizuoka, Japan.)
|
| Journal | Japanese journal of pharmacology
(Jpn J Pharmacol)
Vol. 57
Issue 2
Pg. 137-45
(Oct 1991)
ISSN: 0021-5198 JAPAN |
| PMID | 1812295
(Publication Type: Journal Article)
|
| Chemical References |
- Benzazepines
- Biological Factors
- Free Radicals
- Lipid Peroxides
- Thiazoles
- KF 14363
- Carbon Tetrachloride
- Oxygen
|
| Topics |
- Animals
- Ascitic Fluid
(cytology, metabolism)
- Benzazepines
(therapeutic use)
- Biological Factors
(pharmacology)
- Carbon Tetrachloride
(metabolism)
- Erythrocyte Membrane
(drug effects)
- Erythrocytes
(drug effects, metabolism)
- Free Radicals
- Lipid Peroxidation
(drug effects)
- Lipid Peroxides
(biosynthesis)
- Liver
(drug effects, metabolism)
- Liver Diseases
(metabolism, prevention & control)
- Male
- Oxygen
(metabolism)
- Rats
- Rats, Inbred Strains
- Thiazoles
(therapeutic use)
|
