Abstract |
The down-regulation of CD62L that accompanies T lymphocyte activation is thought to redirect cells away from lymph nodes to sites of infection. In this study, CD62L was maintained on Ag-activated T cells and their distribution, and ability to clear pathogen from peripheral sites determined. CD62L was down-regulated on Ag-specific CD8 T cells in lungs of C57BL/6 mice but maintained in CD62L transgenic mice at day 8 after influenza infection. However, the numbers of influenza-specific CD8 T cells recruited were similar in CD62L transgenic and C57BL/6 mice. Memory CD8 T cell numbers in the lungs and noninvolved organs 100 days after primary infection were similar in CD62L transgenic and C57BL/6 mice, despite differing CD62L expression. Transgenic mice expressing wild-type CD62L cleared a recombinant vaccinia virus expressing an influenza-derived CD8 T cell epitope as efficiently as C57BL/6 mice. However, transgenic mice expressing a protease resistant mutant of CD62L showed significantly delayed viral clearance, despite normal CTL generation and the presence of CD107a and IFN-gamma expressing influenza-specific CD8 T cells. These results demonstrate that CD62L down-regulation is not required for CD8 memory cells to home to sites of infection. However, their ability to clear virus is significantly compromised if CD62L shedding is abrogated.
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Authors | Hannah Richards, M Paula Longhi, Kate Wright, Awen Gallimore, Ann Ager |
Journal | Journal of immunology (Baltimore, Md. : 1950)
(J Immunol)
Vol. 180
Issue 1
Pg. 198-206
(Jan 01 2008)
ISSN: 0022-1767 [Print] United States |
PMID | 18097020
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Epitopes, T-Lymphocyte
- Lysosomal-Associated Membrane Protein 1
- L-Selectin
- Peptide Hydrolases
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Topics |
- Animals
- Down-Regulation
- Epitopes, T-Lymphocyte
(genetics, immunology)
- Female
- Humans
- Immunologic Memory
- Influenza, Human
(immunology)
- L-Selectin
(analysis, genetics, metabolism)
- Lung
(immunology)
- Lymphocyte Activation
- Lysosomal-Associated Membrane Protein 1
(metabolism)
- Mice
- Mice, Inbred C57BL
- Mice, Transgenic
- Ovary
(immunology)
- Peptide Hydrolases
(chemistry)
- T-Lymphocytes, Cytotoxic
(immunology)
- Vaccinia virus
(genetics)
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