Mycobacterium bovis BCG induces high mobility group box 1 protein release from monocytic cells.

Abstract

UNLABELLED:

High mobility group box 1 protein (HMGB-1), a nuclear protein is a critical cytokine that mediates the response to infection, injury and inflammation. The aim of our study was to elaborate a reliable in vitro model to investigate whether Mycobacterium bovis BCG is able to induce HMGB-1 secretion from the monocytic U-937 cells. Western blot technique was applied for the detection of HMGB-1 from supernatants of cells, following induction with LPS, Staphylococcus aureus, and Mycobacterium bovis BCG. HMGB-1 was subjected to MALDI-TOF mass and PSD analysis. Quantitation of the secreted HMGB-1 was performed by ELISA. The BCG strain induced higher amounts of secreted HMGB-1 than LPS or Staphylococcus aureus. The translocation of the HMGB-1 to the cytoplasm following infection of cells with BCG was demonstrated by immunofluorescence examinations.

CONCLUSION:

Our pilot experiments draw attention the to HMGB-1-inducing ability of Mycobacterium bovis. Assessment of the pathophysiological role of this late cytokine in mycobacterial infections demands further in vitro and in vivo examinations.

• Authors: Peter Hofner
• Journal: Acta microbiologica et immunologica Hungarica (Acta Microbiol Immunol Hung) Vol. 54 Issue 4 Pg. 323-37 (Dec 2007) ISSN: 1217-8950 [Print] Hungary
• PMID: 18088007 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• HMGB1 Protein

Topics

• Amino Acid Sequence
• Blotting, Western
• Cell Nucleus (metabolism)
• Cytoplasm (metabolism)
• Enzyme-Linked Immunosorbent Assay
• HMGB1 Protein (analysis, metabolism)
• Humans
• Mass Spectrometry
• Molecular Sequence Data
• Mycobacterium bovis
• Protein Transport
• U937 Cells