| Abstract | OBJECTIVE: To study the modulatory effect of hydrogen sulfide (H(2)S) on oxidative stress in the development of pulmonary hypertension induced by hypoxia. METHODS:Twenty male Wistar rats were randomly divided into control group (n=6), hypoxic group (n=6) and hypoxia+NaHS group (n=8). Hypoxic challenge was performed everyday for 21 days. NaHS solution was injected intra-peritoneally everyday before hypoxic challenge for rats in the hypoxia+NaHS group. After 21 days of hypoxia, the mean pulmonary artery pressure(mPAP) was measured by cardiac catheterization. The weight ratio of right ventricle to left ventricle+septum [RV/(LV+SP)] was also measured. The lung homogenates were assayed for total antioxidant capacity(T-AOC), superoxide dismutase (SOD), oxidized glutathione (GSSG), reduced glutathione (GSH), malondiadehyde(MDA) and hydroxy radical(*OH), and the SOD mRNA levels were assayed by real time polymerse chain reaction. RESULTS: After three weeks of hypoxic disposure, hypoxic hypertension and vascular remodeling developed. Compared with the control group, the mPAP[(23.7+/-2.2) mm Hg vs (16.3+/-3.7) mm Hg,P<0.01] and the weight ratio of RV/(LV+SP) increased (P<0.01), but lung tissue T-AOC was decreased by 21.4% (P<0.01).But GSSG was increased by 68.5% (P<0.01) as compared with those of the control rats. However, compared with those of the hypoxic group, the mPAP in rats of hypoxia+NaHS group was decreased [(16.3+/-2.8) mm Hg vs (23.7 +/-2.2) mm Hg]. Administration of NaHS increased T-AOC by 18.8% (P<0.05) but eliminated GSSG by 23.2% (P<0.05) in rats of hypoxia+NaHS group as compared with the hypoxic group. There were no significant changes in lung tissue SOD mRNA level and its capacity among the three groups. CONCLUSION: Hydrogen sulfide acted as antioxidant during the oxidative stress of hypoxic pulmonary hypertension, and the mechanism was partly through attenuating the content of GSSG. |
| Authors | Hong Ling Wei, Jun Bao Du, Chao Shu Tang
(Affiliation: Department of Pediatrics, Peking University First Hospital, Beijing 100034, China.)
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| Journal | Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences
(Beijing Da Xue Xue Bao)
Vol. 39
Issue 6
Pg. 565-9
(Dec 18 2007)
ISSN: 1671-167X China |
| PMID | 18087542
(Publication Type: English Abstract, Journal Article)
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