Abstract | BACKGROUND AND STUDY AIMS: Lymphatic infiltration has been recognized as a significant risk factor for lymph node metastasis of submucosal invasive colorectal cancer (SICC), but it is difficult to detect microscopically on hematoxylin and eosin (H&E)-stained slides. We therefore identified lymphatic infiltration of tumor cells with D2-40 monoclonal antibody, which reacts specifically against the endothelium of lymphatic vessels, to make an objective and precise diagnosis. PATIENTS AND METHODS: The surgical specimens of 122 consecutive patients with nonpedunculated SICC were examined for lymphatic infiltration by immunohistochemical staining with D2-40 monoclonal antibody (LI-D) and for venous infiltration by Elastica van Gieson staining (VI-E). RESULTS:
Lymph node metastasis was found in 20 patients. Multivariate analysis showed that LI-D (p = 0.0415) and VI-E (p = 0.0119) were significant risk factors for lymph node metastasis. Regardless of the presence of risk factors including at least either lymphatic infiltration or venous infiltration, no lymph node metastasis-positive patients were found (0%) among the 25 patients whose colorectal cancer had a submucosal invasive depth of less than 1,500 microm. No lymph node metastasis was found in any of the patients with a depth of submucosal invasion of less than 3,000 microm, who had no risk factors, including LI-D or VI-E. CONCLUSIONS: Correct evaluation of lymphatic infiltration by immunohistochemical staining with D2-40 monoclonal antibody may play a crucial role in determining whether there are indications for additional treatment in the management of endoscopically resected SICC.
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Authors | Ken Kawaura, Satoshi Fujii, Yukinori Murata, Takahiro Hasebe, Genichiro Ishii, Tohru Itoh, Yasushi Sano, Norio Saito, Atsushi Ochiai |
Journal | Pathobiology : journal of immunopathology, molecular and cellular biology
(Pathobiology)
Vol. 74
Issue 6
Pg. 328-35
( 2007)
ISSN: 1423-0291 [Electronic] Switzerland |
PMID | 18087197
(Publication Type: Journal Article)
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Copyright | (c) 2007 S. Karger AG, Basel. |
Chemical References |
- Antibodies, Monoclonal
- Antibodies, Monoclonal, Murine-Derived
- monoclonal antibody D2-40
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Topics |
- Adenocarcinoma
(pathology)
- Adult
- Aged
- Aged, 80 and over
- Antibodies, Monoclonal
- Antibodies, Monoclonal, Murine-Derived
- Colorectal Neoplasms
(pathology)
- Female
- Humans
- Immunohistochemistry
- Intestinal Mucosa
(pathology)
- Lymphatic Metastasis
(diagnosis)
- Lymphatic Vessels
(pathology)
- Male
- Middle Aged
- Risk Factors
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