| Abstract | OBJECTIVE: To investigate the expression of prostate cancer antigen-1 (PCA-1) in different prostate tissues and analyze its correlation with the clinical parameters of prostate cancer (PCa). METHODS: The expression of PCA-1 mRNA was detected by RT-PCR in the samples from 45 cases of PCa with various clinico-pathologic characteristics, 30 cases of high-grade prostatic intraepithelial neoplasia (HG-PIN), 43 cases of BPH and 39 cases of other carcinoma tissues. The correlation of PCA-1 mRNA expression with the clinical parameters of PCa was statistically analyzed and the PCA-1 expression was examined in different samples by immunohistochemistry. RESULTS: The positive expression rate of PCA-1 mRNA was 88.9% and 60.0% and that of PCA-1 protein was 84.4% and 50.0% in the patients with PCa and HG-PIN, respectively. PCA-1 mRNA and PCA-1 proteins were not expressed in the BPH and other carcinoma tissues. The expression of PCA-1 mRNA was unrelated with the clinical parameters of PCa (P > 0.05). CONCLUSION: It is suggested that PCA-1 is a PCa-specific gene and its expression is unrelated to the clinical parameters of PCa. It might serve as a specific biomarker for the early diagnosis of PCa. |
| Authors | Bing-qian Liu, Yu-dong Wu, Jin-xing Wei, Tong Zhang, Ran-lu Liu, Teng-xiang Ma
(Affiliation: Department of Urology, the First Affiliated Hospital of Zhengzhou University, Zhengzhou, Henan 450052, China. liubq76 at yahoo.com.cn)
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| Journal | Zhonghua nan ke xue = National journal of andrology
(Zhonghua Nan Ke Xue)
Vol. 13
Issue 11
Pg. 997-1001
(Nov 2007)
ISSN: 1009-3591 China |
| PMID | 18077911
(Publication Type: English Abstract, Journal Article)
|
| Chemical References |
- RNA, Messenger
- Prostate-Specific Antigen
|
| Topics |
- Aged
- Aged, 80 and over
- Gene Expression Regulation, Neoplastic
- Humans
- Immunohistochemistry
- Male
- Middle Aged
- Prostate-Specific Antigen
(biosynthesis, genetics)
- Prostatic Neoplasms
(genetics, metabolism, pathology)
- RNA, Messenger
(genetics, metabolism)
- Reverse Transcriptase Polymerase Chain Reaction
|
