| Abstract | OBJECTIVE: To investigate the association between myocardial ADAMTS-1 expression and myocardial fibrosis in coxsackievirus B(3) (CVB(3))-induced acute and chronic murine myocarditis model. METHODS: Balb/c mice were infected with CVB(3) (single injection or monthly injection for 3 months) to establish acute or chronic myocarditis model. Normal controls received equal-volume Eagles minimal essential medium (EMEM) without CVB(3). Hearts were examined at 7 days or 3 months post CVB(3) infection. Heart slides were stained with collagen specific picrosirius red staining and the collagen volume fraction (CVF) was calculated with image analysis software. The expressions of ADAMTS-1 were determined by RT-PCR and immunohistochemistry. RESULTS: Compared with controls, the CVF levels and myocardial expressions of ADAMTS-1 were significant increased in two myocarditis groups, especially in mice with chronic myocarditis (P < 0.01). The increased expression of ADAMTS-1 was located in endochylema as visualized by immunohistochemistry. Myocardial ADAMTS-1 mRNA was positively correlated with CVF in both myocarditis groups (r(7 days) = 0.65, P < 0.05; r(3 months) = 0.73, P < 0.01). CONCLUSIONS: ADAMTS-1 increased in proportion with collagen accumulation in acute and chronic myocarditis, which might play an important role in the development of myocardial fibrosis by modulating the collagen metabolism. |
| Authors | E Shen, Rui-Zhen Chen, Ying-Zhen Yang, Qi Guo, Yong Yu, Yun-Zeng Zou, Hao-Zhu Chen
(Affiliation: Key Laboratory of Viral Heart Diseases, Ministry of Public Health, Shanghai Institute of Cardiovascular Diseases, Zhongshan Hospital, Fudan University, Shanghai 200032, China.)
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| Journal | Zhonghua xin xue guan bing za zhi [Chinese journal of cardiovascular diseases]
(Zhonghua Xin Xue Guan Bing Za Zhi)
Vol. 35
Issue 9
Pg. 854-8
(Sep 2007)
ISSN: 0253-3758 China |
| PMID | 18070483
(Publication Type: English Abstract, Journal Article)
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