[Proteomics study of the serum biomarkers in tongue cancer].

Abstract

OBJECTIVE:

To search for specific serum biomarkers associated with tongue cancer by means of the serum proteomics technology.

METHODS:

The tongue cancer cells of human tongue cancer cell line Tca8113 were subcutaneously inoculated into nude mice, while control nude mice were injected with phosphate-buffered saline. Serums from these two group of mice were collected for proteomic analysis using two-dimensional gel electrophoresis (2-DE) and mass spectrometry (MS).

RESULTS:

Comparing the serum 2-DE maps from the tumor-bearing mice with those produced from control mice, we found that squamous cell carcinoma antigen 1 was over expressed only in the serum of tumor-bearing mice.

CONCLUSIONS:

The squamous cell carcinoma antigen 1 may be of great potential as the biomarker of tongue cancer and as the potential therapeutic target for gene therapy.

• Authors: Xin Huang, Fei-Wu Kang, Long-Jiang Li, Yu-Ming Wen
• Journal: Zhonghua kou qiang yi xue za zhi = Zhonghua kouqiang yixue zazhi = Chinese journal of stomatology (Zhonghua Kou Qiang Yi Xue Za Zhi) Vol. 42 Issue 9 Pg. 550-2 (Sep 2007) ISSN: 1002-0098 [Print] China
• PMID: 18070433 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Biomarkers, Tumor

Topics

• Animals
• Biomarkers, Tumor (blood)
• Carcinoma, Squamous Cell (blood, pathology)
• Cell Line, Tumor
• Electrophoresis, Gel, Two-Dimensional
• Humans
• Mass Spectrometry
• Mice
• Mice, Inbred BALB C
• Mice, Nude
• Proteomics (methods)
• Tongue Neoplasms (blood, pathology)
• Xenograft Model Antitumor Assays