Abstract | AIM: To determine whether Carbon (CO) liberated from CO-releasing molecules attenuates leukocyte infiltration in the small intestine of thermally injured mice. METHODS: Thirty-six mice were assigned to four groups. Mice in the sham group (n = 9) were underwent to sham thermal injury; mice in the burn group (n = 9) received 15% total body surface area full-thickness thermal injury; mice in the burn + CORM-2 group (n = 9) were underwent to the same thermal injury with immediate administration of tricarbonyldichlororuthenium (II) dimer CORM-2 (8 mg/kg, i.v.); and mice in the burn+DMSO group (n = 9) were underwent to the same thermal injury with immediate administration of 160 muL bolus injection of 0.5% DMSO/saline. Histological alterations and granulocyte infiltration of the small intestine were assessed. Polymorphonuclear neutrophil (PMN) accumulation ( myeloperoxidase assay) was assessed in mice mid-ileum. Activation of nuclear factor ( NF)-kappa B, expression levels of intercellular adhesion molecule-1 (ICAM-1) and inducible heme oxygenase in mid-ileum were assessed. RESULTS: Treatment of thermally injured mice with CORM-2 attenuated PMN accumulation and prevented activation of NF-kappa B in the small intestine. This was accompanied by a decrease in the expression of ICAM-1. In parallel, burn-induced granulocyte infiltration in mid-ileum was markedly decreased in the burn mice treated with CORM-2. CONCLUSION: CORM-released CO attenuates leukocyte infiltration in the small intestine of thermally injured mice by interfering with NF-kappa B activation and protein expression of ICAM-1, and therefore suppressing the pro-adhesive phenotype of endothelial cells.
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Authors | Bing-Wei Sun, Qin Jin, Yan Sun, Zhi-Wei Sun, Xi Chen, Zhao-Yong Chen, Gediminas Cepinskas |
Journal | World journal of gastroenterology
(World J Gastroenterol)
Vol. 13
Issue 46
Pg. 6183-90
(Dec 14 2007)
ISSN: 1007-9327 [Print] United States |
PMID | 18069757
(Publication Type: Journal Article)
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Chemical References |
- NF-kappa B
- Organometallic Compounds
- tricarbonyldichlororuthenium (II) dimer
- Intercellular Adhesion Molecule-1
- Carbon
- Carbon Monoxide
- Peroxidase
- Heme Oxygenase (Decyclizing)
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Topics |
- Animals
- Burns
(metabolism)
- Carbon
(metabolism)
- Carbon Monoxide
(metabolism)
- Cell Movement
(drug effects)
- Heat Stress Disorders
(metabolism)
- Heme Oxygenase (Decyclizing)
(metabolism)
- Ileum
(metabolism, pathology)
- Intercellular Adhesion Molecule-1
(metabolism)
- Intestine, Small
(metabolism)
- Male
- Mice
- Mice, Inbred C57BL
- NF-kappa B
(metabolism)
- Neutrophils
(drug effects, pathology)
- Organometallic Compounds
(pharmacology)
- Peroxidase
(metabolism)
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