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Carbon liberated from CO-releasing molecules attenuates leukocyte infiltration in the small intestine of thermally injured mice.

AbstractAIM:
To determine whether Carbon (CO) liberated from CO-releasing molecules attenuates leukocyte infiltration in the small intestine of thermally injured mice.
METHODS:
Thirty-six mice were assigned to four groups. Mice in the sham group (n = 9) were underwent to sham thermal injury; mice in the burn group (n = 9) received 15% total body surface area full-thickness thermal injury; mice in the burn + CORM-2 group (n = 9) were underwent to the same thermal injury with immediate administration of tricarbonyldichlororuthenium (II) dimer CORM-2 (8 mg/kg, i.v.); and mice in the burn+DMSO group (n = 9) were underwent to the same thermal injury with immediate administration of 160 muL bolus injection of 0.5% DMSO/saline. Histological alterations and granulocyte infiltration of the small intestine were assessed. Polymorphonuclear neutrophil (PMN) accumulation (myeloperoxidase assay) was assessed in mice mid-ileum. Activation of nuclear factor (NF)-kappa B, expression levels of intercellular adhesion molecule-1 (ICAM-1) and inducible heme oxygenase in mid-ileum were assessed.
RESULTS:
Treatment of thermally injured mice with CORM-2 attenuated PMN accumulation and prevented activation of NF-kappa B in the small intestine. This was accompanied by a decrease in the expression of ICAM-1. In parallel, burn-induced granulocyte infiltration in mid-ileum was markedly decreased in the burn mice treated with CORM-2.
CONCLUSION:
CORM-released CO attenuates leukocyte infiltration in the small intestine of thermally injured mice by interfering with NF-kappa B activation and protein expression of ICAM-1, and therefore suppressing the pro-adhesive phenotype of endothelial cells.
AuthorsBing-Wei Sun, Qin Jin, Yan Sun, Zhi-Wei Sun, Xi Chen, Zhao-Yong Chen, Gediminas Cepinskas
JournalWorld journal of gastroenterology (World J Gastroenterol) Vol. 13 Issue 46 Pg. 6183-90 (Dec 14 2007) ISSN: 1007-9327 [Print] United States
PMID18069757 (Publication Type: Journal Article)
Chemical References
  • NF-kappa B
  • Organometallic Compounds
  • tricarbonyldichlororuthenium (II) dimer
  • Intercellular Adhesion Molecule-1
  • Carbon
  • Carbon Monoxide
  • Peroxidase
  • Heme Oxygenase (Decyclizing)
Topics
  • Animals
  • Burns (metabolism)
  • Carbon (metabolism)
  • Carbon Monoxide (metabolism)
  • Cell Movement (drug effects)
  • Heat Stress Disorders (metabolism)
  • Heme Oxygenase (Decyclizing) (metabolism)
  • Ileum (metabolism, pathology)
  • Intercellular Adhesion Molecule-1 (metabolism)
  • Intestine, Small (metabolism)
  • Male
  • Mice
  • Mice, Inbred C57BL
  • NF-kappa B (metabolism)
  • Neutrophils (drug effects, pathology)
  • Organometallic Compounds (pharmacology)
  • Peroxidase (metabolism)

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