In order to study the roles of
integrin beta6 in Foot-and-Mouth Disease Virus
infection, pig
integrin beta6 was firstly molecularly cloned from
RNA of the tongue and lung of recovered pig infected experimentally with foot-and-mouth-disease virus (FMDV), and was compared with the beta6 gene of other animals available in GenBank at
nucleotide and
amino acid leves. GeneBank association number of the beta6 gene is EF432729. Pig
integrin beta6 gene (2367bp) encodes a
polypeptide of 788
amino acids consisting of 9 potential N-linked glycosylation sites, 3
Glycosaminoglycan attachment sites, a
cGMP-dependent protein kinase phosphorylation site, 10
Protein kinase C phosphorylation sites, 2
EGF-like domains and 2
cysteine-rich regions. Pig
integrin beta6 subunit has a 26-residue putative
signal peptide, a 681-residue ectodomain, a 29-residue transmembrane domain, and a 52-residue cytoplasmic domain. 11 mutant
nucleotides were found in beta6 gene coding region and 9
amino acids were changed. The nucleotide sequence similarity of
integrin beta6 gene between rheses monkey, mouse, Norway rat, dog, guinea pig, human, bovine, sheep is 79.5%, 84.9%, 85.4%, 85.2%, 88.7%, 90.1%, 91.9% and 91.9%, and the amino acid sequence similarity is 93.5%, 88.2%, 88.5%, 88.3%, 91.0%, 92.8%, 93.3% and 93.4% respectively. This study will lay a foundation for understanding the interactions of FMDV with receptors.