The expression of IL-20 and IL-24 and their shared receptors are increased in rheumatoid arthritis and spondyloarthropathy.

Abstract

The purpose of this study was to analyze the expression of the two proinflammatory cytokines IL-20 and IL-24 and their shared receptors in rheumatoid arthritis and spondyloarthropathy. IL-20 was increased in plasma of rheumatoid arthritis patients compared with osteoarthritis patients and IL-24 was increased in synovial fluid and plasma of rheumatoid arthritis and spondyloarthropathy patients compared with osteoarthritis patients. IL-20 and IL-24 mRNA was only present at low levels in the synovium. In the synovial membrane, IL-20 protein was present in mononuclear cells and neutrophil granulocytes whereas IL-24 protein was observed in endothelial cells and mononuclear cells. IL-20 receptor type 1 and IL-22 receptor were expressed by granulocytes in the synovial fluid. In synovial fluid mononuclear cell cultures, stimulation with recombinant human IL-20 or recombinant human IL-24 induced monocyte chemoattractant protein 1 (CCL2/MCP-1) secretion, but not tumour necrosis factor alpha mRNA synthesis or IL-6 secretion. Both IL-20 and IL-24 showed correlations to CCL2/MCP-1 in plasma from rheumatoid arthritis and spondyloarthropathy patients. This study associates IL-20 and IL-24 to the synovium of rheumatoid arthritis and spondyloarthropathy and results indicate that the two cytokines contribute to disease pathogenesis through recruitment of neutrophil granulocytes and induction of CCL2/MCP-1.

• Authors: Tue Wenzel Kragstrup, Kristian Otkjaer, Christian Holm, Annette Jørgensen, Marianne Hokland, Lars Iversen, Bent Deleuran
• Journal: Cytokine (Cytokine) Vol. 41 Issue 1 Pg. 16-23 (Jan 2008) ISSN: 1096-0023 [Electronic] England
• PMID: 18061474 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• CCL2 protein, human
• Chemokine CCL2
• Interleukins
• Receptors, Interleukin
• interleukin-20 receptor
• interleukin-24
• interleukin 20

Topics

• Adult
• Aged
• Arthritis, Rheumatoid (metabolism, pathology)
• Cells, Cultured
• Chemokine CCL2 (biosynthesis)
• Endothelial Cells (metabolism, pathology)
• Female
• Humans
• Interleukins (biosynthesis, pharmacology)
• Leukocytes, Mononuclear (pathology)
• Male
• Middle Aged
• Neutrophils (metabolism, pathology)
• Osteoarthritis (metabolism, pathology)
• Receptors, Interleukin (biosynthesis)
• Spondylarthropathies (metabolism, pathology)
• Synovial Fluid (metabolism)