| Abstract | This study reports the effect of the fat-soluble vitamin A or vitamin E and grape seed proanthocyanidin extract (GSPE) on oxidative DNA damage estimated by 8-oxo-7, 8-dihydro-2'-deoxyguanosine (8-oxodG) contents in urine and leukocyte of rats. Little is known about the antioxidant potency of dietary anthocyanidins and consequently, the aim of this study was to establish whether anthocyanidins could act as putative antioxidant micronutrients. Seven groups of male Sprague-Dawley rats were fed during 47 days with the following diets: a basic diet, two deficient vitamin A or E diets, two supplemented vitamin A or E diets and two supplemented diets enriched with two doses of grape seed proanthocyanidin extract. At the end of the diet intervention period, 24h, urine and blood were collected. The levels of 8-oxodG in leukocytes rats were significantly lower in the supplemented vitamin A, E and GSPE diet groups with respect to the control group. However, consumption of alpha-tocopherol, vitamin A or GSPE had no effect on the excretion of the oxidised nucleoside 8-oxodG. These results suggest that a vitamin E and A and GSPE enriched-diets have a protective effect on oxidative DNA damage limited to rat leukocytes. |
| Authors | Bénédicte Morin, Jean-François Narbonne, Daniel Ribera, Carine Badouard, Jean-Luc Ravanat
(Affiliation: Laboratoire de Physico-Toxicochimie des Systèmes Naturels, UMR 5472, CNRS Université Bordeaux I, 33405 Talence Cedex, France. b.morin at ism.u-bordeaux.fr)
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| Journal | Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association
(Food Chem Toxicol)
Vol. 46
Issue 2
Pg. 787-96
(Feb 2008)
ISSN: 0278-6915 England |
| PMID | 18060676
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
| Chemical References |
- Antioxidants
- Plant Extracts
- Proanthocyanidins
- Vitamins
- grape seed proanthocyanidins
- Vitamin A
- Vitamin E
- 8-oxo-7-hydrodeoxyguanosine
- Deoxyguanosine
|
| Topics |
- Animals
- Antioxidants
(pharmacology)
- DNA Damage
(drug effects)
- Deoxyguanosine
(analogs & derivatives, blood, urine)
- Diet
- Leukocytes
(drug effects, metabolism)
- Male
- Plant Extracts
(pharmacology)
- Proanthocyanidins
(pharmacology)
- Rats
- Rats, Sprague-Dawley
- Vitamin A
(pharmacology)
- Vitamin E
(pharmacology)
- Vitamins
(pharmacology)
|
