| Abstract | We aimed to detect the protective effect of trapidil in ischemia-reperfusion (IR) injury due to ovarian torsion and detorsion. Thirty-two pubertal New Zealand albino rabbits were used. Adnexal torsion was created by rotating the left adnexa including the tubal and ovarian vessels in a 360 degrees clockwise direction. Adnexal detorsion was done by untwisting the adnexa. In the IR group, left oopherectomy was performed after 3 h of adnexal torsion and 3 h of adnexal detorsion. In the study group, a 3-h adnexal torsion was performed and trapidil was administered intraperitoneally as a single dose of 40 mg/kg, 1 h before detorsion. The left oopherectomy was performed after a 3-h adnexal detorsion. In the sham group, sham operation was performed followed by left oopherectomy. In the control group, normal ovarian tissue was evaluated. Catalase, superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and malondialdehyde (MDA) levels of ovarian tissue were determined for each group. The values of SOD and GSH-Px activities in the IR group were significantly decreased (P < 0.05). In addition, the MDA level was significantly higher in the IR group (P < 0.01). The trapidil-administered group showed significant increase in the levels of GSH-Px (P < 0.05), catalase (P < 0.05), SOD (P < 0.05), and decreased MDA levels (P < 0.05) compared to those in the IR group. The study has shown that trapidil treatment prevents ischemia induced oxidative damage in the ovarian tissues of rabbits. |
| Authors | Salih Somuncu, Murat Cakmak, Gunnur Dikmen, Hülya Akman, Murat Kaya
(Affiliation: Department of Pediatric Surgery, School of Medicine, Kirikkale University, 71100 Kirikkale, Turkey. somuncusal at yahoo.com)
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| Journal | Pediatric surgery international
(Pediatr Surg Int)
Vol. 24
Issue 3
Pg. 315-8
(Mar 2008)
ISSN: 0179-0358 Germany |
| PMID | 18060415
(Publication Type: Journal Article)
|
| Chemical References |
- Vasodilator Agents
- Trapidil
- Malondialdehyde
- Catalase
- Glutathione Peroxidase
- Superoxide Dismutase
|
| Topics |
- Animals
- Catalase
(metabolism)
- Female
- Glutathione Peroxidase
(metabolism)
- Lipid Peroxidation
- Malondialdehyde
(metabolism)
- Ovary
(blood supply, metabolism)
- Rabbits
- Reperfusion Injury
(etiology, prevention & control)
- Superoxide Dismutase
(metabolism)
- Torsion Abnormality
(complications)
- Trapidil
(pharmacology)
- Vasodilator Agents
(pharmacology)
|
