c-Maf expression in angioimmunoblastic T-cell lymphoma.

Abstract

The oncogene c-Maf was recently found to be overexpressed in approximately 50% of multiple myeloma cases, and a role for c-Maf in promoting cyclin D2 expression has been postulated. We previously examined c-Maf expression in various T-cell lymphomas by reverse-transcription polymerase chain reaction and found extremely elevated c-Maf levels in angioimmunoblastic T-cell lymphoma (AILT). In this study, we examined T-cell lymphomas for c-Maf and cyclin expression immunohistochemically. Of 93 cases of T-cell lymphomas we investigated in the current study, c-Maf expression was seen in 23 out of 31 cases of AILT, 3 out of 11 of adult T-cell leukemia/lymphoma, 4 out of 19 of peripheral T-cell lymphoma, unspecified [PTCL(U)], and 0 out of 11 cases of mycosis fungoides, 0 out of 11 of anaplastic large cell lymphoma, and 1 out of 10 of extranodal NK/T-cell lymphoma, nasal type. Double immunostaining in AILT revealed that the majority of c-Maf-positive cells were also positive for CD43 (MT1), CD45RO (UCHL-1), and CD4 but were negative for CD20 (L26). Additionally, cyclins D1 and D2, which stimulate cell cycle progression, were overexpressed in a large number of the c-Maf-positive AILT samples. Quantitative reverse-transcription polymerase chain reaction analysis also showed that c-Maf was overexpressed in 8/31 cases of AILT, 0/19 cases of PTCL(U), 0/11 cases of anaplastic large cell lymphoma, 0/10 cases of extranodal NK/T-cell lymphoma, nasal type, and 2/8 cases of multiple myeloma, presenting significant difference between AILT and PTCL(U) (P=0.016, chi test). These findings strongly suggest that CD4-positive neoplastic T cells in AILT show c-Maf expression and provide new insight into the pathogenesis of AILT suggesting c-Maf to be a useful diagnostic marker for AILT.

• Authors: Yoshiko Idate Murakami, Yasushi Yatabe, Teruhiro Sakaguchi, Eiichi Sasaki, Yoriko Yamashita, Naoki Morito, Keigyou Yoh, Yuuki Fujioka, Fumihiko Matsuno, Hiroyuki Hata, Hiroaki Mitsuya, Shigehiko Imagawa, Atsushi Suzuki, Hiroyasu Esumi, Masaharu Sakai, Satoru Takahashi, Naoyoshi Mori
• Journal: The American journal of surgical pathology (Am J Surg Pathol) Vol. 31 Issue 11 Pg. 1695-702 (Nov 2007) ISSN: 0147-5185 [Print] United States
• PMID: 18059226 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antigens, CD20
• Biomarkers, Tumor
• CCND2 protein, human
• CD4 Antigens
• Cyclin D
• Cyclin D2
• Cyclins
• Leukosialin
• MAF protein, human
• Proto-Oncogene Proteins c-maf
• RNA, Messenger
• SPN protein, human
• Leukocyte Common Antigens

Topics

• Adult
• Antigens, CD20 (analysis)
• Biomarkers, Tumor (analysis, genetics)
• CD4 Antigens (analysis)
• Cyclin D
• Cyclin D2
• Cyclins (analysis)
• Humans
• Immunoblastic Lymphadenopathy (genetics, metabolism, pathology)
• Immunohistochemistry
• Leukocyte Common Antigens (analysis)
• Leukosialin (analysis)
• Lymphoma, T-Cell (chemistry, genetics, pathology)
• Proto-Oncogene Proteins c-maf (analysis, genetics)
• RNA, Messenger (analysis)
• Reverse Transcriptase Polymerase Chain Reaction
• Up-Regulation