| Abstract | Transcription factors known as CCAAT enhancer binding proteins (C/EBPs) are involved in hematopoietic differentiation, including myelopoiesis and granulopoiesis. C/EBPbeta-deficient mice develop normally; however, they exhibit defective macrophage function, resulting in increased susceptibility to infection. Little is known about the role of C/EBPbeta in granulopoiesis; therefore, we examined granulopoiesis in C/EBPbeta-deficient mice. Morphology, the number of peripheral blood and bone marrow cells, and the expression of genes specific for the myeloid lineage were normal in C/EBPbeta-deficient mice. Interestingly, the hematopoietic progenitor cells of C/EBPbeta-deficient mice did not respond normally to granulocyte/macrophage-colony stimulating factor and granulocyte colony stimulating factor. In addition, C/EBPbeta-deficient neutrophils displayed enhanced apoptosis compared with wild-type neutrophils. Our present results indicate that C/EBPbeta helps regulate survival of neutrophils, downstream of the granulocyte colony stimulating factor receptor. |
| Authors | Tadayuki Akagi, Takayuki Saitoh, James O'Kelly, Shizuo Akira, Adrian F Gombart, H Phillip Koeffler
(Affiliation: Division of Hematology and Oncology, Cedars-Sinai Medical Center, University of California Los Angeles School of Medicine, Los Angeles, CA 90048, USA. akagit at cshs.org)
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| Journal | Blood
(Blood)
Vol. 111
Issue 6
Pg. 2999-3004
(Mar 15 2008)
ISSN: 0006-4971 United States |
| PMID | 18056834
(Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't)
|
| Chemical References |
- Antigens, Surface
- CCAAT-Enhancer-Binding Protein-beta
- Cytokines
- RNA, Messenger
- Granulocyte Colony-Stimulating Factor
- Fluorouracil
- Granulocyte-Macrophage Colony-Stimulating Factor
|
| Topics |
- Animals
- Antigens, Surface
(immunology)
- Apoptosis
(drug effects)
- Bone Marrow Cells
(cytology, immunology, metabolism)
- CCAAT-Enhancer-Binding Protein-beta
(deficiency, genetics, metabolism)
- Cell Lineage
- Cytokines
(genetics)
- Fluorouracil
(pharmacology)
- Gene Expression Regulation
- Granulocyte Colony-Stimulating Factor
(pharmacology)
- Granulocyte-Macrophage Colony-Stimulating Factor
(pharmacology)
- Hematopoiesis
(drug effects)
- Leukocytes
(cytology, immunology)
- Mice
- Mice, Knockout
- RNA, Messenger
(genetics)
|
