| Abstract | Specific primers and TaqMan MGB probes were designed with Primer Express 2.0 software according to the conserved region of the H5, H9, H7 subtype AIV hemagglutinin gene to make research of real-time fluorescent one-step PCR in the differential detection of H5, H9, H7 subtype avian influenza inactivated vaccines. The result showed that the method was specific and reproducible. No cross-reaction was discovered with other avian disease vaccines. Real-time fluorescent PCR provided a specific, sensitive, rapid and convenient method for the subtype identification of avian influenza inactivated vaccines. |
| Authors | Jian-Feng Han, Yi-Bao Ning, Li Song, Cheng-Huai Yang
(Affiliation: Key Laboratory of Veterinary Drug Innovation and Biosafety Evaluation, Ministry of Agriculture, China Institute of Veterinary Drug Control, Beijing 100081, China.)
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| Journal | Sheng wu gong cheng xue bao = Chinese journal of biotechnology
(Sheng Wu Gong Cheng Xue Bao)
Vol. 23
Issue 5
Pg. 953-7
(Sep 2007)
ISSN: 1000-3061 China |
| PMID | 18051881
(Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)
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