Methamphetamine modulates DC-SIGN expression by mature dendritic cells.

Abstract

We report that methamphetamine (meth) may act as cofactor in human immunodeficiency virus (HIV)-1 pathogenesis by increasing dendritic cell (DC)-specific intercellular adhesion molecule-3 (ICAM-3) grabbing non-integrin (DC-SIGN) expression on DCs. Mature DCs (MDCs), obtained from normal subjects, cultured with meth show an up-regulation of DC-SIGN gene and protein expression as analyzed by real-time quantitative polymerase chain reaction and fluorescence-activated cell-sorting analyses, respectively. Furthermore, these meth-induced effects were reversed by a dopamine D1 receptor antagonist (SCH 23390) and small interfering RNA specific to the D1 receptor (D1R) demonstrating that meth-induced effects are mediated through these receptors. Furthermore, meth in synergy with the HIV-1 peptide gp120 up-regulates DC-SIGN gene expression by MDCs. These data are the first evidence that meth up-regulates the expression of DC-SIGN on MDCs. A better understanding of the role of DC-SIGN in HIV-1 infection may help to design novel therapeutic strategies against the progression of HIV-1 disease in the drug-using population.

• Authors: Madhavan P N Nair, Supriya Mahajan, Don Sykes, Meghana V Bapardekar, Jessica L Reynolds
• Journal: Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology (J Neuroimmune Pharmacol) Vol. 1 Issue 3 Pg. 296-304 (Sep 2006) ISSN: 1557-1904 [Electronic] United States
• PMID: 18040806 (Publication Type: Journal Article)

Chemical References

• Cell Adhesion Molecules
• Central Nervous System Stimulants
• DC-specific ICAM-3 grabbing nonintegrin
• HIV Envelope Protein gp120
• Lectins, C-Type
• RNA, Small Interfering
• Receptors, Cell Surface
• Receptors, Dopamine D1
• gp120 protein, Human immunodeficiency virus 1
• Methamphetamine

Topics

• Cell Adhesion Molecules (biosynthesis, drug effects)
• Central Nervous System Stimulants (pharmacology)
• Dendritic Cells (drug effects)
• Flow Cytometry
• Gene Expression (drug effects)
• HIV Envelope Protein gp120 (pharmacology)
• Humans
• Lectins, C-Type (biosynthesis, drug effects)
• Methamphetamine (pharmacology)
• RNA, Small Interfering
• Receptors, Cell Surface (biosynthesis, drug effects)
• Receptors, Dopamine D1 (antagonists & inhibitors)
• Reverse Transcriptase Polymerase Chain Reaction
• Transfection