High performance thin layer chromatographic analysis of neutral lipids in the urine of BALB/c mice infected with Echinostoma caproni.

Abstract

Silica gel high performance thin layer chromatography-densitometry was used to determine the neutral lipid profile in the urine of BALB/c mice experimentally infected with adults of Echinostoma caproni. An approximate 30 worm burden per host induced certain changes in the neutral lipid profile of the mice at 2 to 10 weeks post-infection (PI) relative to the uninfected controls. Infection caused a significant increase (Student's t test, P < 0.05) in the methyl oleate fraction at 6 and 7 weeks PI relative to the uninfected controls. Neutral lipid profiles of urine may serve as an indicator of experimental infection of echinostomes in mice.

• Authors: James D Vasta, Bernard Fried, Joseph Sherma
• Journal: Parasitology research (Parasitol Res) Vol. 102 Issue 4 Pg. 625-9 (Mar 2008) ISSN: 0932-0113 [Print] Germany
• PMID: 18040717 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Lipids
• Oleic Acids
• methyl oleate

Topics

• Animals
• Chromatography, High Pressure Liquid
• Echinostoma (pathogenicity)
• Echinostomiasis (parasitology, urine)
• Female
• Lipids (analysis, isolation & purification)
• Mice
• Mice, Inbred BALB C
• Oleic Acids (analysis)
• Urine (chemistry)