Abstract | AIMS/HYPOTHESIS: METHODS: Subcutaneous adipose tissue mRNA expression of D5D (also known as FADS1), D6D (also known as FADS2) and SCD was determined in 75 individuals representative of the study population of 294 healthy 63-year-old men. Desaturation indexes (product/substrate fatty acid ratios) were generated from adipose tissue fatty acid composition in all individuals. Insulin resistance was defined as the upper quartile of the updated homeostasis model assessment (HOMA-2) index. RESULTS: The relevant desaturation indexes (16:1/16:0, 18:1/18:0, 20:4/20:3 and 18:3/18:2) reflected expression of SCD, but not of D5D or D6D in adipose tissue. Insulin-resistant individuals had a higher adipose tissue 18:1/18:0, but not 16:1/16:0 ratio than insulin-sensitive individuals. Individuals with a high adipose tissue 18:1/18:0 ratio were 4.4-fold (95% CI 1.8-11.8) more likely to be insulin resistant [threefold (95% CI 1.1-8.6) after adjustment for waist circumference and plasma triacylglycerol]. In a multiple regression model predicting HOMA-2, the independent effect of the 18:1/18:0 ratio was borderline (p=0.086). CONCLUSIONS/INTERPRETATION: Adipose tissue desaturation indexes of SCD reflect the expression of the gene encoding the enzyme in this tissue. Elevated SCD activity within adipose tissue is closely coupled to the development of insulin resistance.
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Authors | P Sjögren, J Sierra-Johnson, K Gertow, M Rosell, B Vessby, U de Faire, A Hamsten, M-L Hellenius, R M Fisher |
Journal | Diabetologia
(Diabetologia)
Vol. 51
Issue 2
Pg. 328-35
(Feb 2008)
ISSN: 0012-186X [Print] Germany |
PMID | 18030445
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Delta-5 Fatty Acid Desaturase
- RNA, Messenger
- Fatty Acid Desaturases
- Stearoyl-CoA Desaturase
- FADS1 protein, human
- Linoleoyl-CoA Desaturase
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Topics |
- Adipose Tissue
(enzymology, metabolism)
- Delta-5 Fatty Acid Desaturase
- Fatty Acid Desaturases
(genetics, metabolism)
- Gene Expression Regulation, Enzymologic
- Humans
- Insulin Resistance
- Linoleoyl-CoA Desaturase
(genetics, metabolism)
- Male
- Middle Aged
- RNA, Messenger
(genetics, metabolism)
- Stearoyl-CoA Desaturase
(genetics, metabolism)
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