Abstract | PURPOSE: METHODS:
mRNA and protein expression was evaluated by quantitative real-time RT-PCR and Western blotting, respectively. Humoral immune response to recombinant tankyrases was investigated by modified enzyme-linked immunoassays. Cellular immune response was analysed by ELISPOT and (51)Cr release assays. RESULTS: We found that both mRNA and protein levels of tankyrase 2 (TNKL) are upregulated in colon tumors. In contrast, protein level of tankyrase 1 (TNKS) is downregulated, while mRNA level shows variable changes. More than a quarter of colon cancer patients develop humoral immune response to at least one of the two tankyrases. In this study we mapped common and unique B-cell epitopes located in different domains of the two proteins. Additionally, we present evidence for T-cell responses both to epitopes that are unique for TNKL and to those shared between TNKL and TNKS. CONCLUSION:
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Authors | Yuriy V Shebzukhov, Inna N Lavrik, Julia Karbach, Svetlana V Khlgatian, Ekaterina P Koroleva, Pavel V Belousov, Kirill N Kashkin, Alexander Knuth, Elke Jager, Nai-Wen Chi, Dmitry V Kuprash, Sergei A Nedospasov |
Journal | Cancer immunology, immunotherapy : CII
(Cancer Immunol Immunother)
Vol. 57
Issue 6
Pg. 871-81
(Jun 2008)
ISSN: 0340-7004 [Print] Germany |
PMID | 18026951
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Biomarkers, Tumor
- Epitopes
- TNKS2 protein, human
- Tankyrases
- TNKS protein, human
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Topics |
- Amino Acid Sequence
- Antibody Formation
- Biomarkers, Tumor
(metabolism)
- CD8-Positive T-Lymphocytes
(immunology, metabolism)
- Colonic Neoplasms
(enzymology, pathology)
- Epitope Mapping
(methods)
- Epitopes
(chemistry)
- Humans
- Immunity, Cellular
- Immunotherapy
(methods)
- Molecular Sequence Data
- Neoplasms
(immunology, therapy)
- Sequence Homology, Amino Acid
- Tankyrases
(biosynthesis, metabolism)
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