Abstract | OBJECTIVES/HYPOTHESIS: STUDY DESIGN: Prospective animal-based research study. METHODS: Fifteen (n = 15) rats were made allergic via sensitization to ovalbumin (OVA) by subcutaneous injection and randomized into three groups: saline (SAL) + LPS, olopatadine (OLO) + LPS, and azelastine (AZE) + LPS. Allergic rats were transtympanically injected with OVA 24 hours prior to challenge, creating a subclinical inflammatory response in which there is no visible middle ear effusion. Thirty-five microliters of test substance (SAL + LPS, OLO + LPS, or AZE + LPS) were injected into the middle ear at 0, 2, and 4 hours. Effusion was collected at 2, 4, and 6 hours. Statistical analysis was performed on effusion volume and albumin concentration. RESULTS: Significant increase in effusion volume with respect to time was noted for the SAL + LPS group, whereas the AZE + LPS group demonstrated a decrease. Intergroup comparison revealed a significant decrease in effusion volume at hour 6, with both AZE and OLO less than LPS alone. A significant decrease in albumin concentration over time was noted in the AZE group. Intergroup comparison revealed a significant difference inalbumin concentration at hour 6, with OLO significantly lower than LPS. CONCLUSIONS: Ototopical antihistamines are effective in reducing effusion volume and albumin concentration in LPS-induced otitis media in an allergic rat model. This finding supports the hypothesis that ototopic antihistamines may prove effective in treating patients with allergy-induced eustachian tube dysfunction.
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Authors | Jeffrey L Cutler, Robert F Labadie |
Journal | The Laryngoscope
(Laryngoscope)
Vol. 118
Issue 2
Pg. 283-7
(Feb 2008)
ISSN: 0023-852X [Print] United States |
PMID | 18025947
(Publication Type: Evaluation Study, Journal Article)
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Chemical References |
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Topics |
- Animals
- Disease Models, Animal
- Food Hypersensitivity
(prevention & control)
- Histamine H1 Antagonists
(pharmacology, therapeutic use)
- Hypersensitivity
(complications, drug therapy)
- Otitis Media with Effusion
(complications, drug therapy)
- Prospective Studies
- Rats
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