| Abstract | The present paper reported on an anti-CCA monoclonal antibody, McAb-IIID 10, which could be used in determinations of both parasite-oriented circulating antigens and specific anti-CCA antibodies. The established competitive ELISA (C-ELISA) using McAb-IIID 10 to detect schistosome-antibodies showed high sensitivity and specificity in the diagnosis of schistosomiasis with few cross-reactions. In field trials, coincident rates in 3 separate batches of serum samples when subjected to double-blind detections were obtained. A total of 1,915 serum samples had been determined by C-ELISA, among them 113 acute cases achieved a 100% positive rate, 765 chronic and 25 late cases showed 96.3% and 72% positive respectively. 70% of the 66 cured schistosomiasis cases turned to be negative. None of the 750 normal individuals showed positive reactions. No cross reaction was found in 27 sera from hydatidosis, whereas 1 and 2 positive reactions were found in 43 paragonimiasis sera and 126 clonorchiasis sera respectively. The established McAb-IIID 10 involved Dot-ELISA was found of value in the assessment of effective chemotherapy and showed a high negative conversion rate of 97.9% in 48 cured schistosomiasis patients. In 16 experimentally infected rabbits, 12 became negative in Dot-ELISA determinations at the 8th week post treatment, and the remaining 4 treated ones, the titer as well as the reaction intensity were also found reduced. A good coincidence rate was also found between C-ELISA and Dot-ELISA, their detection results may be complementary each other. |
| Authors | W X Lou, Z L Qian, C L Xue, A Z Yu, J Y Xu, H Y Zheng, S J Yang, X S Su, L Sheng
(Affiliation: Department of Parasitology, Shanghai Second Medical University.)
|
| Journal | Zhongguo ji sheng chong xue yu ji sheng chong bing za zhi = Chinese journal of parasitology & parasitic diseases
(Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi)
Vol. 9
Issue 4
Pg. 254-7
( 1991)
ISSN: 1000-7423 CHINA |
| PMID | 1802447
(Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)
|
| Chemical References |
- Antibodies, Helminth
- Antibodies, Monoclonal
- Antigens, Helminth
|
| Topics |
- Animals
- Antibodies, Helminth
(blood)
- Antibodies, Monoclonal
(biosynthesis, diagnostic use)
- Antigens, Helminth
(blood)
- Enzyme-Linked Immunosorbent Assay
(methods)
- Humans
- Rabbits
- Schistosoma japonicum
(immunology)
- Schistosomiasis japonica
(diagnosis)
|
