[In vivo study on influence of a discrete nano-hydroxyapatite on leukemia P388 tissue in BALB/C mice].

Abstract

OBJECTIVE:

To study the influence of a discrete nano-hydroxyapatite crystal (nano-HAp) on lymphatic leukemia P388 behavior by in vivo techniques.

METHODS:

A nano-HAp was prepared by a neutralization reaction of 0.1 mol calcium hydroxide suspension and 0.06 mol phosphoric acid solutions at room temperature over pH7. The various doses of the nano-HAp only and the nano-HAp mixture with cyclophosphamide (CY) were injected into mice inoculated with solid tumor lymphatic leukemia P388 and dispersed into PRMI 1640 media harvested the leukemia P388 cells. Sixty P388 BALB/C mice were randomly grouped; 36 of them were used as nano-HAp treated groups and 24 mice as the control groups. The leukemia growth in the mice was examined morphologically, histopathologically and under a transmission electron microscope (TEM).

RESULTS:

The nano-HAp was identified as a hydroxyapatite by an X-ray diffractometry (XRD) and a Fourier transform infrared spectroscopy (FTIR). The morphology and sizes were observed under a TEM. The tissue growth inhibition ratio (weight%) of solid lymphatic leukemia P388 bearing mice treated with nano-HAp at doses 35 mg/kg, 53 mg/kg and nano-HAp (53 mg/kg) combined with CY (35 mg/kg) in 3 consecutive days via intraperitineal injections were 14.95%, 32.67% and 60.45% respectively. Apoptosis of P388 cell cocultured with nano-HAp was confirmed by TEM.

CONCLUSIONS:

The tissue growth restriction of solid tumor lymphatic leukemia P388 was greater after an injection of nano-HAp only or nano-HAp mixed with CY than that obtained after injection with physiological saline solution as a control (P < 0.01), and the tissue growth restriction of solid tumor after an injection of nano-HAp combined with CY was greater than that obtained after nano-HAp or CY injection only (P < 0.01).

• Authors: Ge Li, Jian-ming Huang, Hideki Aoki, Yan Li, Rong Zhang, Bi-fang Deng
• Journal: Zhonghua er ke za zhi = Chinese journal of pediatrics (Zhonghua Er Ke Za Zhi) Vol. 45 Issue 9 Pg. 692-6 (Sep 2007) ISSN: 0578-1310 [Print] China
• PMID: 18021565 (Publication Type: English Abstract, Journal Article)

Chemical References

• Biocompatible Materials
• Durapatite
• Calcium Hydroxide

Topics

• Animals
• Biocompatible Materials (pharmacology)
• Calcium Hydroxide (chemistry)
• Cell Line, Tumor (drug effects, pathology)
• Durapatite (pharmacology)
• Female
• Leukemia P388 (pathology)
• Male
• Mice
• Mice, Inbred BALB C
• Nanoparticles (chemistry)
• X-Ray Diffraction (methods)
• X-Rays