Expression of cell-surface antigens and embryonic stem cell pluripotency genes in equine blastocysts.

Abstract

Embryonic stem-like (ES-like) cells have now been derived from the inner cell mass (ICM) of horse embryos at the blastocyst stage. Because they have been shown to express cell-surface antigens found in both human and mouse ES cells, the present study investigated gene expression patterns in day-7 horse blastocysts from which the horse ES-like cells had been derived originally. The genes studied included Oct-4, stage-specific embryonic antigen-1 (SSEA-1), SSEA-3, SSEA-4, tumor rejection antigen-1-60 (TRA-1-60), TRA-1-81, and alkaline phosphatase activity, and whereas all three of the SSEA antigens were expressed in both the ICM and the trophoblast on day 7, Oct-4, TRA-1-60, TRA-1-81, and alkaline phosphatase activity were localized mostly in the ICM. Upon in vitro differentiation of the horse ES-like cells, their expression of the stem cell markers was abolished. Therefore, the species-specific expression pattern of stem cell markers in horse ES-like cells reflects gene expression in the blastocysts from which they are derived.

• Authors: D J Guest, W R Allen
• Journal: Stem cells and development (Stem Cells Dev) Vol. 16 Issue 5 Pg. 789-96 (Oct 2007) ISSN: 1547-3287 [Print] United States
• PMID: 17999600 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antigens, Surface
• Biomarkers
• Eosine Yellowish-(YS)
• Hematoxylin

Topics

• Animals
• Antigens, Surface (genetics, metabolism)
• Biomarkers (metabolism)
• Blastocyst (cytology, metabolism)
• Cell Differentiation
• Embryonic Stem Cells (cytology, metabolism)
• Eosine Yellowish-(YS)
• Equidae (genetics)
• Gene Expression Regulation
• Hematoxylin
• Immunohistochemistry
• Organ Specificity
• Pluripotent Stem Cells (metabolism)