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Presence of bacterial-DNA in cirrhosis identifies a subgroup of patients with marked inflammatory response not related to endotoxin.

AbstractBACKGROUND/AIMS:
Serum lipopolysaccharide-binding protein and bacterial-DNA have been proposed as markers of bacterial translocation and this study aimed to evaluate the immune response registered by bacterial-DNA from Gram-positive and Gram-negative microorganisms and the effect on lipopolysaccharide-binding protein, to further investigate both markers.
METHODS:
Thirty-two patients were distributed into two groups according to the presence of bacterial-DNA, determined by broad-range PCR of 16SrRNA gene. Serum endotoxin, lipopolysaccharide-binding protein, cytokines and nitric oxide products were measured by ELISA.
RESULTS:
Serum endotoxin and lipopolysaccharide-binding protein were non-significantly higher in patients with bacterial-DNA than in those without bacterial-DNA. Regarding patients with bacterial-DNA from Gram-positive microorganisms (n = 8), these levels were similar to those in patients without bacterial-DNA (n = 16), and significantly lower than in patients with bacterial-DNA from Gram-negative bacteria. Tumour necrosis factor-alpha and interleukin-6 were significantly increased in patients with vs without bacterial-DNA (324.93+/-70.76 vs 134.91+/-34.58microg/mL; p<0.05; 294.96+/-87.48 vs 175.92+/-60.58microg/mL, p < 0.05, respectively). Patients with bacterial-DNA from Gram-positive microorganisms also showed significantly higher levels for both cytokines than patients without bacterial-DNA, and similar to those in patients with bacterial-DNA from Gram-negative bacteria.
CONCLUSIONS:
Patients with translocation of bacterial-DNA from Gram-positive microorganisms showed increased proinflammatory cytokines unrelated to endotoxin, which would not be detected by serum lipopolysaccharide-binding protein measurement.
AuthorsJosé M González-Navajas, Pablo Bellot, Rubén Francés, Pedro Zapater, Carlos Muñoz, Juan Carlos García-Pagán, Sonia Pascual, Miguel Pérez-Mateo, Jaime Bosch, José Such
JournalJournal of hepatology (J Hepatol) Vol. 48 Issue 1 Pg. 61-7 (Jan 2008) ISSN: 0168-8278 [Print] England
PMID17998145 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Acute-Phase Proteins
  • Biomarkers
  • Carrier Proteins
  • Cytokines
  • DNA, Bacterial
  • Endotoxins
  • Interleukin-6
  • Lipopolysaccharides
  • Membrane Glycoproteins
  • Tumor Necrosis Factor-alpha
  • lipopolysaccharide-binding protein
  • Nitric Oxide
Topics
  • Acute-Phase Proteins
  • Aged
  • Bacterial Translocation
  • Biomarkers
  • Carrier Proteins (blood)
  • Cytokines (blood)
  • DNA, Bacterial (analysis)
  • Endotoxins (toxicity)
  • Enzyme-Linked Immunosorbent Assay
  • Female
  • Gram-Negative Bacteria (chemistry)
  • Gram-Positive Bacteria (chemistry)
  • Humans
  • Inflammation (diagnosis, pathology)
  • Interleukin-6 (blood)
  • Lipopolysaccharides (blood)
  • Liver (chemistry, pathology)
  • Liver Cirrhosis (diagnosis, pathology)
  • Male
  • Membrane Glycoproteins (blood)
  • Middle Aged
  • Nitric Oxide (blood)
  • Tumor Necrosis Factor-alpha (blood)

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