| Abstract | Expression of the pro-inflammatory cytokine interleukin-1 beta (IL-1beta) is increased following the nervous system injury. Generally IL-1beta induces inflammation, leading to neural degeneration, while several neuropoietic effects have also been reported. Although neurite outgrowth is an important step in nerve regeneration, whether IL-1beta takes advantages on it is unclear. Now we examine how it affects neurite outgrowth. Following sciatic nerve injury, expression of IL-1beta is increased in Schwann cells around the site of injury, peaking 1 day after injury. In dorsal root ganglion (DRG) neurons and cerebellar granule neurons (CGNs), neurite outgrowth is inhibited by the addition of myelin-associated glycoprotein (MAG), activating RhoA. IL-1beta overcomes MAG-induced neurite outgrowth inhibition, by deactivating RhoA. Intracellular signaling experiments reveal that p38 MAPK, and not nuclear factor-kappa B (NF-kappaB), mediated this effect. These findings suggest that IL-1beta may contribute to nerve regeneration by promoting neurite outgrowth following nerve injury. |
| Authors | Ko Temporin, Hiroyuki Tanaka, Yusuke Kuroda, Kiyoshi Okada, Koji Yachi, Hisao Moritomo, Tsuyoshi Murase, Hideki Yoshikawa
(Affiliation: Department of Orthopaedics, Osaka University Graduate School of Medicine, Suita, Osaka 565-0871, Japan.)
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| Journal | Biochemical and biophysical research communications
(Biochem Biophys Res Commun)
Vol. 365
Issue 2
Pg. 375-80
(Jan 11 2008)
ISSN: 1090-2104 United States |
| PMID | 17996195
(Publication Type: Journal Article)
|
| Chemical References |
- Interleukin-1beta
- p38 Mitogen-Activated Protein Kinases
- rhoA GTP-Binding Protein
|
| Topics |
- Animals
- Cells, Cultured
- Dose-Response Relationship, Drug
- Enzyme Activation
(drug effects)
- Female
- Interleukin-1beta
(administration & dosage)
- MAP Kinase Signaling System
(physiology)
- Nerve Regeneration
(drug effects, physiology)
- Neurites
(drug effects, physiology, ultrastructure)
- Posterior Horn Cells
(cytology, drug effects, physiology)
- Rats
- Rats, Wistar
- p38 Mitogen-Activated Protein Kinases
(metabolism)
- rhoA GTP-Binding Protein
(metabolism)
|
