[Preparation and identification of monoclonal antibodies against snake venom C-type lectin like protein Agkisacutacin].

Abstract

AIM:

To prepare and identify the antibodies against snake venom C-type lectin like protein Agkisacutacin.

METHODS:

A BALB/c mouse was immunized with Agkisacutacin and the cells were fused by standard hybridoma technique to prepare mAbs. The stability of the hybridomas secreting mAbs was detected by indirect ELISA. The nuclear type of the hybridomas was analyzed by fluorescent staining and the specificity of mAbs was detected by Western blot.

RESULTS:

Three cell strains of hybridomas that could steadily secrete anti-Agkisacutacin mAb were obtained.

CONCLUSION:

The successful preparation of anti-Agkisacutacin mAbs provides an important tool for studying the in vivo metabolism of new anti-thrombus drugs by detecting Agkisacutacin and investigating the mechanism of anti-thrombus of the drugs.

• Authors: Li-Jing Su, Liang Xiao, Tao Zhan, Lan Chen, Yan-Qiu Dong, Li Fang, Lian-Sheng Cheng, Jing Liu
• Journal: Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology (Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi) Vol. 23 Issue 11 Pg. 1031-3 (Nov 2007) ISSN: 1007-8738 [Print] China
• PMID: 17988585 (Publication Type: Journal Article)

Chemical References

• Antibodies, Monoclonal
• Crotalid Venoms
• Snake Venoms
• agkisacutacin

Topics

• Animals
• Antibodies, Monoclonal (analysis, immunology, metabolism)
• Antibody Specificity
• Crotalid Venoms (biosynthesis, immunology, isolation & purification)
• Enzyme-Linked Immunosorbent Assay
• Female
• Hybridomas (metabolism)
• Mice
• Mice, Inbred BALB C
• Snake Venoms (chemistry, immunology)