Abstract | BACKGROUND: MATERIALS AND METHODS: Rats were randomly allocated to five groups of IIR induced by occlusion of the superior mesenteric artery for 45 min followed by 1 min, 2, 4, and 8 h of reperfusion (expGroups) and five corresponding sham groups (sGroups). Bronchoalveolar lavage fluid was obtained from the right lung and its biochemical ( protein, PLA(2), PAF-AcH) and cytological characteristics were determined. Plasma malonyldialdehyde was measured as a marker of lipid peroxidation. The 4 and 8 h reperfusion expGroups had significantly (P < 0.05) elevated alveolar-arterial O(2) gradient values compared with the corresponding controls. Total protein, PLA(2) and PAF-AcH levels significantly (P < 0.05) increased in expGroups compared with the corresponding shams after 4 h of reperfusion. Total bronchoalveolar lavage fluid cells and plasma malonyldialdehyde were significantly (P < 0.05) elevated in expGroups compared with the sGroups after 2 h of reperfusion. CONCLUSIONS: PLA(2) could act synergistically or parallel with the reactive oxygen species produced during IIR, resulting in the induction or even in the exacerbation of the inflammatory reaction in acute respiratory distress syndrome. PAF-AcH could play an anti-inflammatory role by reducing the concentration of PAF.
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Authors | Georgia Kostopanagiotou, Efthimios Avgerinos, Constantinos Costopanagiotou, Nikolaos Arkadopoulos, Ioanna Andreadou, Kalliopi Diamantopoulou, Marilena Lekka, Vassilios Smyrniotis, George Nakos |
Journal | The Journal of surgical research
(J Surg Res)
Vol. 147
Issue 1
Pg. 108-16
(Jun 01 2008)
ISSN: 0022-4804 [Print] United States |
PMID | 17981297
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Platelet Activating Factor
- Reactive Oxygen Species
- Malondialdehyde
- Phospholipases A2
- 1-Alkyl-2-acetylglycerophosphocholine Esterase
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Topics |
- 1-Alkyl-2-acetylglycerophosphocholine Esterase
(physiology)
- Animals
- Bronchoalveolar Lavage Fluid
(cytology)
- Disease Models, Animal
- Intestines
(blood supply)
- Lung
(pathology)
- Male
- Malondialdehyde
(blood)
- Phospholipases A2
(physiology)
- Platelet Activating Factor
(physiology)
- Pulmonary Gas Exchange
- Rats
- Rats, Wistar
- Reactive Oxygen Species
(metabolism)
- Reperfusion Injury
(etiology)
- Respiratory Distress Syndrome
(etiology)
- Time Factors
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