| Abstract | The aim of this study was to clarify the effects of the muscarinic receptor agonist, cevimeline, on saliva flow and expression of aquaporin5 (AQP5) in submandibular gland after X-ray irradiation. Using a previously established radiation-induced xerostomia model mouse, saliva flow from at 7 days before irradiation to at 28 days after irradiation was investigated in mice that were treated with cevimeline before or after irradiation. Radiation caused a significant decrease in saliva flow compared with nonirradiated salivary glands. Cevimeline post-treatment also caused a significant decrease in saliva flow. In contrast, cevimeline pre-treatment did not significantly decrease saliva flow. Expression of AQP5 fluorescent intensity and mRNA were also analyzed. Irradiation significantly decreased expression of AQP5 in submandibular gland. However, pre-treatment with cevimeline prevented this decrease in AQP5 expression. These data suggest that pretreatment with cevimeline prevents radiation-induced xerostomia and radiation-induced decrease in expression of AQP5 in submandibular gland. |
| Authors | Katsuhiro Takakura, Sachiko Takaki, Ienaka Takeda, Nobuyuki Hanaue, Yasuhiro Kizu, Morio Tonogi, Gen-yuki Yamane
(Affiliation: Department of Oral Medicine, Oral and Maxillofacial Surgery, Tokyo Dental Collage, Ichikawa, Chiba, Japan.)
|
| Journal | The Bulletin of Tokyo Dental College
(Bull Tokyo Dent Coll)
Vol. 48
Issue 2
Pg. 47-56
(May 2007)
ISSN: 0040-8891 Japan |
| PMID | 17978545
(Publication Type: Comparative Study, Journal Article)
|
| Chemical References |
- Aqp5 protein, mouse
- Aquaporin 5
- Muscarinic Agonists
- Quinuclidines
- Thiophenes
- cevimeline
|
| Topics |
- Animals
- Aquaporin 5
(analysis, drug effects, radiation effects)
- Disease Models, Animal
- Female
- Fluorescent Antibody Technique
- Mice
- Mice, Inbred ICR
- Muscarinic Agonists
(therapeutic use)
- Quinuclidines
(therapeutic use)
- Radiation Dosage
- Reverse Transcriptase Polymerase Chain Reaction
- Saliva
(drug effects, radiation effects, secretion)
- Secretory Rate
(drug effects, radiation effects)
- Submandibular Gland
(radiation effects)
- Submandibular Gland Diseases
(drug therapy, etiology)
- Thiophenes
(therapeutic use)
- Time Factors
- X-Rays
- Xerostomia
(drug therapy, etiology)
|
