Detection of HHV-8 and HPV in laryngeal carcinoma.

Abstract

OBJECTIVE:

In this study we aimed to investigate the presence of human herpesvirus 8 (HHV-8) and human papillomavirus (HPV) in laryngeal carcinoma.

MATERIALS AND METHODS:

Fifty patients operated on because of laryngeal carcinoma were included in the study. Forty-seven had squamous cell carcinoma (SCC) whereas three had verrucous carcinoma. Fresh tumoral tissues, or tumoral tissues obtained from archival paraffin-embedded blocks, were examined. HHV-8 DNA and HPV DNA were detected using polymerase chain reaction (PCR) and viral genotypes of HPV were determined via the hybrid capture method. The presence of HHV-8 DNA and HPV DNA were also investigated in normal appearing laryngeal tissue collected from 50 cadavers at autopsy.

RESULTS:

HPV DNA was detected in seven patients (7/50; 14%) (5 out of 47 patients with SCC (5/47; 10.6%) and two out of three patients with verrucous carcinoma). HHV-8 DNA was detected in five patients and they all had SCC (5/47; 10.6%). One case had both HHV-8 and HPV DNA. None of the control samples from cadavers harbored HHV-8 DNA, or HPV DNA. There was a statistically significant correlation between HHV-8 DNA and HPV DNA positivity and laryngeal SCC (Fisher exact test; p=0.023 for each). No statistically significant correlations were found between the presence of HHV-8 and/or HPV and age, gender, tumor stage, differentiation, the site of the tumor, smoking and alcohol use.

CONCLUSIONS:

The findings of the present study suggest that beside HPV, HHV-8 might have a role in laryngeal carcinogenesis. Further investigations are necessary to clarify the exact role of these viruses in laryngeal carcinoma.

• Authors: Melih Güven Güvenç, Kenan Midilli, Ahmet Ozdoğan, Ender Inci, Rauf Tahamiler, Ozgün Enver, Gözde Sirin, Sevgi Ergin, Mert Kuşkucu, Emel Ozergil Divanoğlu, Gülden Yilmaz, Kemal Altas
• Journal: Auris, nasus, larynx (Auris Nasus Larynx) Vol. 35 Issue 3 Pg. 357-62 (Sep 2008) ISSN: 0385-8146 [Print] Netherlands
• PMID: 17977679 (Publication Type: Journal Article)

Chemical References

• DNA, Viral

Topics

• Adult
• Aged
• Carcinoma, Squamous Cell (pathology, virology)
• Carcinoma, Verrucous (pathology, virology)
• DNA, Viral (genetics)
• Female
• Genotype
• Herpesvirus 8, Human (genetics)
• Humans
• Laryngeal Neoplasms (pathology, virology)
• Larynx (pathology, virology)
• Male
• Middle Aged
• Neoplasm Staging
• Papillomaviridae (genetics)
• Polymerase Chain Reaction