The
antidiabetic and antioxidative effect of
osajin was monitored under the conditions of
alloxan-induced
diabetes mellitus in an in vivo experiment. The animals were divided by random selection into 2 groups (n=7). The treated group was administered
osajin in peroral doses of 10 mg/kg in
Avicel, the placebo diabetic group was given only the
solution of
Avicel, and the last group was intact. Selected laboratory parameters (
glucose,
urea,
cholesterol, antioxidative
enzymes, total antioxidative capacity,
malondialdehyde in serum, diuresis, total
glucose and
protein losses through urine) were determined in all animals. Kidney tissue and pancreas samples were taken for histopathological analysis. The findings included a statistically significant increase (p< or =0.05) in the
glutathione peroxidase catalytic activity, total antioxidative capacity (p< or =0.01) and a statistically significant decrease (p< or =0.01) on
malondialdehyde level in the treated group compared to the placebo group. A statistically highly significant decrease (p< or =0.01) in diuresis,
glucose and
protein losses through urine were identified in the treated group compared to the placebo group. The
superoxide dismutase catalytic activity,
urea and
cholesterol levels involved non-significant changes. The results of biochemical examination show a protective
antidiabetic and antioxidative effect of
osajin. The results of histopathological examination correlate with them only partially.