Abstract | BACKGROUND: METHODS AND RESULTS: The vascular response to reactive hyperemia, assessed by measuring postischemic increase in forearm arterial compliance, and the plasma concentration of soluble cell adhesion molecules were evaluated in 21 adult apoA-I(M) carriers, 21 age- and gender-matched nonaffected relatives (control subjects), and 21 healthy subjects with low HDL-C (low-HDL subjects). The average plasma HDL-C and apoA-I levels of apoA-I(M) carriers were remarkably lower than those of control subjects and significantly lower than those of low-HDL subjects. The postischemic increase in forearm arterial compliance in the apoA-I(M) carriers was 2-fold greater than in low-HDL subjects and remarkably similar to that of control subjects. Plasma soluble cell adhesion molecule levels were similar in apoA-I(M) carriers and control subjects but were greater in low-HDL subjects. When incubated with endothelial cells, HDL isolated from apoA-I(M) carriers was more effective than HDL from control and low-HDL subjects in stimulating endothelial nitric oxide synthase expression and activation and in downregulating tumor necrosis factor-alpha-induced expression of vascular cell adhesion molecule-1. CONCLUSIONS: Despite their very low HDL levels, apoA-I(M) carriers do not display typical features of impaired vascular function because of an improved activity of apoA-I(M) HDL in maintaining endothelial cell homeostasis.
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Authors | Monica Gomaraschi, Damiano Baldassarre, Mauro Amato, Sonia Eligini, Paola Conca, Cesare R Sirtori, Guido Franceschini, Laura Calabresi |
Journal | Circulation
(Circulation)
Vol. 116
Issue 19
Pg. 2165-72
(Nov 06 2007)
ISSN: 1524-4539 [Electronic] United States |
PMID | 17967773
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Apolipoprotein A-I
- Lipoproteins, HDL
- Vascular Cell Adhesion Molecule-1
- Intercellular Adhesion Molecule-1
- Nitric Oxide
- NOS3 protein, human
- Nitric Oxide Synthase Type III
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Topics |
- Adult
- Aged
- Apolipoprotein A-I
(blood, genetics)
- Cells, Cultured
- Compliance
- Endothelium, Vascular
(cytology, physiology)
- Female
- Forearm
(blood supply)
- Heterozygote
- Homeostasis
(physiology)
- Humans
- Hyperemia
(physiopathology)
- Intercellular Adhesion Molecule-1
(blood)
- Lipoproteins, HDL
(blood)
- Male
- Middle Aged
- Mutation
- Nitric Oxide
(metabolism)
- Nitric Oxide Synthase Type III
(genetics, metabolism)
- Regional Blood Flow
(physiology)
- Umbilical Veins
(cytology)
- Vascular Cell Adhesion Molecule-1
(blood, genetics)
- Vasodilation
(physiology)
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