Oral vaccination with different antigens from Yersinia pestis KIM delivered by live attenuated Salmonella typhimurium elicits a protective immune response against plague.

Abstract	The use of live recombinant Salmonella attenuated vaccine (RASV) encoding Yersinia proteins is a promising new approach for the vaccination against Yersinia pestis. We have tested the efficacy of 2 proteins, Psn and a portion of LcrV in protecting mice against virulent Yersinia pestis challenge. To remove the immunosuppressive properties of LcrV protein, the lcrV gene, without the TLR2 receptor sequence, was cloned into a beta-lactamase secretion vector. Immunizations were performed with RSAV expressing LcrV or Psn. Challenge with a virulent Y. pestis strain was performed 4 weeks after the last immunization. Our results show that the truncated LcrV protein delivered by RASV is sufficient to afford a full protective immune response in a mouse model of bubonic plague and the Psn protein afforded partial protection in a non-optimized system. This finding should facilitate the design and development of a new generation of vaccines against Y. pestis.
Authors	Christine G Branger, Jacqueline D Fetherston, Robert D Perry, Roy Curtiss 3rd
Journal	Advances in experimental medicine and biology (Adv Exp Med Biol) Vol. 603 Pg. 387-99 ( 2007) ISSN: 0065-2598 [Print] United States
PMID	17966435 (Publication Type: Journal Article, Research Support, N.I.H., Extramural)
Chemical References	Antibodies, Bacterial Antigens, Bacterial DNA, Bacterial LcrV protein, Yersinia Plague Vaccine Pore Forming Cytotoxic Proteins Recombinant Proteins Vaccines, Attenuated Vaccines, Synthetic
Topics	Administration, Oral Animals Antibodies, Bacterial (biosynthesis) Antigens, Bacterial (genetics, immunology) Base Sequence DNA, Bacterial (genetics) Female Genes, Bacterial Mice Mice, Inbred BALB C Plague (immunology, prevention & control) Plague Vaccine (administration & dosage, genetics, immunology) Pore Forming Cytotoxic Proteins (genetics, immunology) Recombinant Proteins (genetics, immunology) Salmonella typhimurium (genetics, immunology) Vaccines, Attenuated (administration & dosage, genetics, immunology) Vaccines, Synthetic (administration & dosage, genetics, immunology) Yersinia pestis (genetics, immunology)

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