| Abstract | Mortality from plague is high if not treated with the proper antibiotics within 18-24 hours after onset of symptoms. The process of antibiotic susceptibility determination of Yersinia pestis isolated from blood samples may extend from 4 to more than 7 days, since the in vitro growth is very slow. To accelerate this process, we developed an enrichment protocol as well as a non-standard yet reliable method for rapid antibiotic susceptibility analysis of Y. pestis from blood cultures using flow cytometry technology. This rapid method is applicable to blood cultures containing low levels of Y. pestis. |
| Authors | Ida Steinberger-Levy, Eran Zahavy, Sara Cohen, Yehuda Flashner, Emanuelle Mamroud, Moshe Aftalion, David Gur, Raphael Ber
(Affiliation: Department of Biochemistry and Molecular Genetics, Israel Institute for Biological Research, Ness-Ziona.)
|
| Journal | Advances in experimental medicine and biology
(Adv Exp Med Biol)
Vol. 603
Pg. 339-50
( 2007)
ISSN: 0065-2598 United States |
| PMID | 17966430
(Publication Type: Journal Article)
|
| Topics |
- Bacteremia
(diagnosis, drug therapy, microbiology)
- Bacteriological Techniques
- Blood
(microbiology)
- Colony Count, Microbial
- Flow Cytometry
- Humans
- Microbial Sensitivity Tests
- Plague
(diagnosis, drug therapy, microbiology)
- Yersinia pestis
(drug effects, growth & development, isolation & purification)
|
