Abstract | OBJECTIVES: METHODS: All rabbits were subjected to 60 minutes ischemia by left anterior descending coronary artery occlusion (LADO) and 6 hours reperfusion. The rabbits are randomly divided into 3 groups (n = 8 in each group): (1) Ischemia-reperfusion (IR): LADO and reperfusion without additional intervention; (2) RI-Post: after 60 minutes of LADO, the left renal artery was occluded for 30 seconds and reperfused for 30 seconds and repeated 3 times, then the coronary artery was reperfused for 6 hours; (3) Medication intervention (MI): 10 minutes before coronary reperfusion, rabbits were treated with PKC antagonist GF109203X (0.05 mg/kg, IV), followed by RI-Post treatment and 6 hours coronary reperfusion. Myocardial apoptosis was measured by TUNEL and the myocardial Bcl-2 and Bax protein expressions were assessed by immunohistochemistry. RESULTS: Compared with the IR group and the MI group, myocardial apoptosis was significantly reduced (P < 0.05) and the Bcl-2 protein expression increased (P < 0.01) while the Bax protein expression decreased (P < 0.05) in the RI-Post group. CONCLUSIONS: Remote renal postconditioning applied right before the onset of coronary artery reperfusion can reduce the myocardial apoptosis induced by myocardial ischemia and reperfusion and up-regulate Bcl-2 while down-regulate Bax expression possibly by activation of protein kinase C.
|
Authors | Song Liu, Xiang-feng Wu, Wen-zhong Zhang, Ying-xian Sun, Shang-lang Cai |
Journal | Zhonghua xin xue guan bing za zhi
(Zhonghua Xin Xue Guan Bing Za Zhi)
Vol. 35
Issue 8
Pg. 757-60
(Aug 2007)
ISSN: 0253-3758 [Print] China |
PMID | 17963639
(Publication Type: English Abstract, Journal Article)
|
Chemical References |
- Proto-Oncogene Proteins c-bcl-2
- bcl-2-Associated X Protein
|
Topics |
- Animals
- Apoptosis
- Female
- Ischemia
(metabolism)
- Ischemic Preconditioning
- Kidney Diseases
(metabolism)
- Male
- Myocardial Reperfusion Injury
(metabolism)
- Myocardium
(metabolism)
- Proto-Oncogene Proteins c-bcl-2
(metabolism)
- Rabbits
- bcl-2-Associated X Protein
(metabolism)
|