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[Remote postconditioning by brief renal ischemia and reperfusion reduces acute myocardial ischemia and reperfusion induced myocardial apoptosis in rabbits].

AbstractOBJECTIVES:
To observe the effects of renal ischemic postconditioning (RI-Post) on myocardial apoptosis in rabbits with acute myocardial ischemia and reperfusion.
METHODS:
All rabbits were subjected to 60 minutes ischemia by left anterior descending coronary artery occlusion (LADO) and 6 hours reperfusion. The rabbits are randomly divided into 3 groups (n = 8 in each group): (1) Ischemia-reperfusion (IR): LADO and reperfusion without additional intervention; (2) RI-Post: after 60 minutes of LADO, the left renal artery was occluded for 30 seconds and reperfused for 30 seconds and repeated 3 times, then the coronary artery was reperfused for 6 hours; (3) Medication intervention (MI): 10 minutes before coronary reperfusion, rabbits were treated with PKC antagonist GF109203X (0.05 mg/kg, IV), followed by RI-Post treatment and 6 hours coronary reperfusion. Myocardial apoptosis was measured by TUNEL and the myocardial Bcl-2 and Bax protein expressions were assessed by immunohistochemistry.
RESULTS:
Compared with the IR group and the MI group, myocardial apoptosis was significantly reduced (P < 0.05) and the Bcl-2 protein expression increased (P < 0.01) while the Bax protein expression decreased (P < 0.05) in the RI-Post group.
CONCLUSIONS:
Remote renal postconditioning applied right before the onset of coronary artery reperfusion can reduce the myocardial apoptosis induced by myocardial ischemia and reperfusion and up-regulate Bcl-2 while down-regulate Bax expression possibly by activation of protein kinase C.
AuthorsSong Liu, Xiang-feng Wu, Wen-zhong Zhang, Ying-xian Sun, Shang-lang Cai
JournalZhonghua xin xue guan bing za zhi (Zhonghua Xin Xue Guan Bing Za Zhi) Vol. 35 Issue 8 Pg. 757-60 (Aug 2007) ISSN: 0253-3758 [Print] China
PMID17963639 (Publication Type: English Abstract, Journal Article)
Chemical References
  • Proto-Oncogene Proteins c-bcl-2
  • bcl-2-Associated X Protein
Topics
  • Animals
  • Apoptosis
  • Female
  • Ischemia (metabolism)
  • Ischemic Preconditioning
  • Kidney Diseases (metabolism)
  • Male
  • Myocardial Reperfusion Injury (metabolism)
  • Myocardium (metabolism)
  • Proto-Oncogene Proteins c-bcl-2 (metabolism)
  • Rabbits
  • bcl-2-Associated X Protein (metabolism)

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