Haeamtang induces apoptosis of colon cancer HT-29 cells through activation of caspase-3.

Abstract

The effect of Haeamtang (HAT) on the colon cancer HT-29 cells was investigated in this study. A water extract of HAT significantly decreased the number of HT-29 cells in a dose-and time-dependent manner as determined by a MTT assay. Flow cytometry results revealed a dose- and time-dependent increase of dead cells in HT-29 cells treated with HAT extract. The anticancer activity of the H AT extract is attributed to apoptosis induced in HT-29 cells, which was demonstrated by increased caspase-3 activity and poly-ADP-ribose polymerase fragmentation. A selective caspase inhibitor, z-VAD-fmk, inhibited the HAT-induced cell death. Taken together, these results demonstrate that HAT extract induces apoptosis in HT-29 cells.

• Authors: Phil-Dong Moon, Hyun-Na Koo, Hyun-Ja Jeong, Ho-Jeong Na, Su-Jin Kim, Gab-Soo Hwang, Na-Ra Han, Seung-Heon Hong, Hyung-Min Kim, Jae-Young Um
• Journal: The American journal of Chinese medicine (Am J Chin Med) Vol. 35 Issue 5 Pg. 897-909 ( 2007) ISSN: 0192-415X [Print] Singapore
• PMID: 17963328 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Haeamtang
• Plant Extracts
• Caspase 3

Topics

• Apoptosis (drug effects)
• Blotting, Western
• Caspase 3 (metabolism)
• Enzyme Activation
• Flow Cytometry
• HT29 Cells
• Humans
• Medicine, Traditional
• Plant Extracts