Abstract | OBJECTIVE: METHODS: Confocal microscopy was used to study the expression of FITC ( fluorescein isothiocyanate)-alpha-BTX, anti-synaptophysin (A-SY) antibody, and anti-neurofilament (A-NF) antibody to determine the distribution of nAChR and ganglion cell and nerve fibres in colon specimens from five cases of HD and three normal controls. RESULTS: Quantitative assessment of the immunoreactivity of colonic muscle and colonic mucosal epithelium from an aganglionic segment of HD bowel demonstrated markedly increased nAChR compared with colonic muscle and colonic mucosal epithelium from a ganglionic segment of HD bowel and normal bowel (p < 0.0001, respectively), both of which have only a few positive nAChR. In colonic muscle from aganglionic and transitional segments of HD, there were many nAChR around hypertrophic nerve trunks identified by A-NF and A-SY staining. CONCLUSION: We suggest that abnormal expression of nAChR in HD might be implicated in causing gastrointestinal dysmotility because of their localization around hypertrophic nerve trunks.
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Authors | Junichi Kusafuka, Hiroyuki Kobayashi, Katsumi Miyahara, Geoffrey J Lane, Noriyoshi Sueyoshi, Atsuyuki Yamataka |
Journal | Asian journal of surgery
(Asian J Surg)
Vol. 30
Issue 4
Pg. 255-60
(Oct 2007)
ISSN: 1015-9584 [Print] China |
PMID | 17962128
(Publication Type: Journal Article)
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Chemical References |
- Bungarotoxins
- Receptors, Nicotinic
- Acetylcholinesterase
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Topics |
- Acetylcholinesterase
(metabolism)
- Bungarotoxins
(metabolism)
- Child, Preschool
- Female
- Gastrointestinal Motility
(physiology)
- Hirschsprung Disease
(metabolism)
- Humans
- Immunohistochemistry
- Infant
- Intestinal Mucosa
(metabolism)
- Male
- Nerve Fibers
(metabolism)
- Receptors, Nicotinic
(metabolism)
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