Selenium methylselenocysteine protects human hepatoma HepG2 cells against oxidative stress induced by tert-butyl hydroperoxide.

Abstract

Selenium methylselenocysteine (Se-MeSeCys) is a common selenocompound in the diet with a tested chemopreventive effect. This study investigated the potential protective effect of Se-MeSeCys against a chemical oxidative stress induced by tert-butyl hydroperoxide (t-BOOH) on human hepatoma HepG2 cells. Speciation of selenium derivatives by liquid chromatography-inductively coupled plasma mass spectrometry depicts Se-MeSeCys as the only selenocompound in the cell culture. Cell viability (lactate dehydrogenase) and markers of oxidative status--concentration of reduced glutathione (GSH) and malondialdehyde (MDA), generation of reactive oxygen species (ROS) and activity of the antioxidant enzymes glutathione peroxidase (GPx) and glutathione reductase (GR)--were evaluated. Pretreatment of cells with Se-MeSeCys for 20 h completely prevented the enhanced cell damage, MDA concentration and GR and GPx activity and the decreased GSH induced by t-BOOH but did not prevent increased ROS generation. The results show that treatment of HepG2 cells with concentrations of Se-MeSeCys in the nanomolar to micromolar range confers a significant protection against an oxidative insult.

• Authors: Susana Cuello, Sonia Ramos, Raquel Mateos, M Angeles Martín, Yolanda Madrid, Carmen Cámara, Laura Bravo, Luis Goya
• Journal: Analytical and bioanalytical chemistry (Anal Bioanal Chem) Vol. 389 Issue 7-8 Pg. 2167-78 (Dec 2007) ISSN: 1618-2650 [Electronic] Germany
• PMID: 17952420 (Publication Type: Journal Article)

Chemical References

• Anticarcinogenic Agents
• Organoselenium Compounds
• Reactive Oxygen Species
• Selenocysteine
• Malondialdehyde
• tert-Butylhydroperoxide
• Glutathione
• Cysteine
• selenomethylselenocysteine

Topics

• Anticarcinogenic Agents (pharmacology)
• Carcinoma, Hepatocellular (metabolism)
• Cell Line, Tumor
• Cell Survival
• Cysteine (analogs & derivatives, pharmacology)
• Glutathione (analysis)
• Humans
• Liver Neoplasms (metabolism)
• Malondialdehyde (analysis)
• Organoselenium Compounds (pharmacology)
• Oxidative Stress (drug effects)
• Reactive Oxygen Species (metabolism)
• Selenocysteine (analogs & derivatives)
• Time Factors
• tert-Butylhydroperoxide (toxicity)