Morin (3,5,7,2',4'-pentahydroxyflavone), a plant-derived
flavonoid belonging to the subclass of
flavonol is believed to play a role in
chemoprevention and
cancer chemotherapy. In this study, we found that the cotreatment of
morin (500 ppm in diet) for 16 weeks to N-nitosodiethylamine-induced (200 mg/kg bodyweight in
drinking water) rats provides protection against the oxidative stress caused by the
carcinogen and thereby prevents hepatocellular
carcinogenesis. On administration of the
carcinogen, the level of lipid peroxidation increased markedly, but was found to be significantly lowered by
morin treatment. On the contrary, the
antioxidant levels in both liver and serum were decreased in
carcinogen-administered animals, which was improved to normalcy upon
morin administration. Cotreatment with
morin prevented the elevation of marker
enzymes induced by
N-nitrosodiethylamine. The
body weight of the animals decreased and their relative liver weight increased significantly on
N-nitrosodiethylamine administration when compared to control group. However, cotreatment with
morin significantly prevented the decrease of the
body weight and increase in relative liver weight caused by DEN. Histological observations of liver tissue too correlated with the biochemical observations. In conclusion, these findings indicate that
morin prevents lipid peroxidation, hepatic cell damage and protects the
antioxidant system in
N-nitrosodiethylamine-induced hepatocellular
carcinogenesis.