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A short treatment of cells with the lanthanide ions La3+, Ce3+, Pr3+ or Nd3+ changes the cellular chemistry into a state in which RNA replication of flaviviruses is specifically blocked without interference with host-cell multiplication.

Abstract
Alpha- and flaviviruses contain class II fusion proteins, which form ion-permeable pores in the target membrane during virus entry. The pores generated during entry of the alphavirus Semliki Forest virus have been shown previously to be blocked by lanthanide ions. Here, analyses of the influence of rare earth ions on the entry of the flaviviruses West Nile virus and Uganda S virus revealed an unexpected effect of lanthanide ions. The results showed that a 30 s treatment of cells with an appropriate lanthanide ion changed the cellular chemistry into a state in which the cells no longer supported the multiplication of flaviviruses. This change occurred in cells treated before, during or after infection, did not inhibit multiplication of Semliki Forest virus and did not interfere with host-cell multiplication. The change was generated in vertebrate and insect cells, and was elicited in the presence of actinomycin D. In vertebrate cells, the change was elicited specifically by La(3+), Ce(3+), Pr(3+) and Nd(3+). In insect cells, additional lanthanide ions had this activity. Further analyses showed that lanthanide ion treatment blocked the ability of the host cell to support the replication of flavivirus RNA. These results open two areas of research: the study of molecular alterations induced by lanthanide ion treatment in uninfected cells and the analysis of the resulting modifications of the flavivirus RNA replicase complex. The findings possibly open the way for the development of a general chemotherapy against flavivirus diseases such as Dengue fever, Japanese encephalitis, West Nile fever and yellow fever.
AuthorsGerd Wengler, Gisela Wengler, Andreas Koschinski
JournalThe Journal of general virology (J Gen Virol) Vol. 88 Issue Pt 11 Pg. 3018-3026 (Nov 2007) ISSN: 0022-1317 [Print] England
PMID17947525 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Antiviral Agents
  • Lanthanoid Series Elements
  • RNA, Viral
Topics
  • Animals
  • Antiviral Agents (pharmacology)
  • Cell Line
  • Cell Proliferation (drug effects)
  • Chlorocebus aethiops
  • Cricetinae
  • Culicidae
  • Flavivirus (growth & development)
  • Lanthanoid Series Elements (pharmacology)
  • RNA, Viral (biosynthesis)
  • Semliki forest virus (growth & development)
  • Viral Plaque Assay
  • Virus Replication (drug effects)

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