| Abstract | 1. Hydrogen sulphide (H(2)S) acts as a gaseous cellular messenger and has recently been reported to induce a suspended animation-like state in mice. The aim of the present study was to investigate the protective role of H(2)S exposure in stress gastric ulcer. 2. In the present study, we used a rat model of water immersion and restraint stress (WRS) to induce the typical stress disease, namely stress gastric ulcer. Rats were treated with WRS for 4 h, with or without pre-exposure to H(2)S (160 p.p.m. H(2)S for 2.5 h). 3. In H(2)S-exposed rats, body temperature was significantly reduced by 2.5C (P < 0.01) and oxygen consumption was reduced by 37.1% (P < 0.01) compared with control rats. Plasma levels of H(2)S were increased by 20.8% (P < 0.01) following pre-exposure. Pre-exposure to H(2)S significantly reduced the gastric ulcer index, from 24 +/- 9 to 9 +/- 2 (P < 0.01), in WRS rats. In addition, WRS increased plasma levels of adrenocorticotropin (ACTH) and corticosterone 4.7- and 4.8-fold, respectively (both P < 0.01). Pre-exposure to H(2)S markedly suppressed plasma ACTH and corticosterone level by 34.4 and 53.2%, respectively (both P < 0.01), and reduced WRS-elevated myeloperoxidase (MPO) activity by 19%. In the present study, WRS increased gastric malondialdehyde and conjugated diene content by 42 and 68%, respectively (both P < 0.01), and H(2)S exposure reduced lipid peroxide production. Finally, H(2)S exposure inhibited the WRS-elevated expression of glucose-regulated protein 78 and caspase 12, markers of endoplasmic reticulum stress. 4. In conclusion, a low concentration of H(2)S may be a new pharmacological tool for induced hypothermia to prevent severe stress-induced diseases and multifarious trauma in the clinical setting. |
| Authors | Li-Xia Lou, Bin Geng, Jun-Bao Du, Chao-Shu Tang
(Affiliation: Institute of Cardiovascular Diseases, Peking University First Hospital, Beijing, China.)
|
| Journal | Clinical and experimental pharmacology & physiology
(Clin Exp Pharmacol Physiol)
Vol. 35
Issue 2
Pg. 223-8
(Feb 2008)
ISSN: 1440-1681 Australia |
| PMID | 17941893
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
| Chemical References |
- Casp12 protein, rat
- Heat-Shock Proteins
- Molecular Chaperones
- molecular chaperone GRP78
- Corticosterone
- Malondialdehyde
- Hydrogen Sulfide
- Adrenocorticotropic Hormone
- Peroxidase
- Caspase 12
|
| Topics |
- Adrenocorticotropic Hormone
(blood)
- Animals
- Body Temperature
(drug effects)
- Caspase 12
(metabolism)
- Corticosterone
(blood)
- Disease Models, Animal
- Endoplasmic Reticulum
(drug effects, metabolism)
- Gastric Mucosa
(drug effects, metabolism, pathology)
- Heat-Shock Proteins
(metabolism)
- Hydrogen Sulfide
(pharmacology, therapeutic use)
- Hypothermia, Induced
(methods)
- Lipid Peroxidation
(drug effects)
- Male
- Malondialdehyde
(metabolism)
- Molecular Chaperones
(metabolism)
- Oxygen Consumption
(drug effects)
- Peroxidase
(metabolism)
- Rats
- Rats, Sprague-Dawley
- Stomach Ulcer
(etiology, metabolism, pathology, prevention & control)
- Stress, Psychological
(complications, metabolism, pathology)
|
