Abstract | OBJECTIVE: METHODS: Premature rats delivered by hysterotomy at 21 days gestation were randomly continuously exposed to moderate hyperoxia (60% FiO2) and room air. The rats were sacrificed at 1, 4, 7, 11 and 14 days of exposure (6 rats at each time point). Lung sections were stained with hematoxylin and eosin for histological examination. Expression of VEGF and eNOS proteins and mRNA were assayed using immunohistochemistry and RT-PCR. RESULTS: After 4 days of hyperoxia, lungs developed interstitial fibrosis, abnormal vascular patterns and decreased alveolar septation. These changes became more obvious with more prolonged hyperoxia exposure. The expression of VEGF protein after 4 and 7 days of exposure decreased significantly in the hyperoxia group compared with controls. The expression of VEGF mRNA in the hyperoxia group was also lower after 4 and 7 days of exposure. Both VEGF protein and mRNA levels decreased with increasing hyperoxia exposure time. The expressions of eNOS protein and mRNA also progressively decreased with increasing hyperoxia exposure. CONCLUSIONS:
Hyperoxia caused progressive reduction in lung VEGF and eNOS expression as well as abnormalities of lung structures, including decreased vascular growth and impaired alveolarization. These histologic changes are similar to those of BPD. The data support a link between BPD and decreased expression of VEGF and eNOS.
|
Authors | Wei Wei, Wei Wang, Qin Ning, Xiao-Ping Luo |
Journal | Zhongguo dang dai er ke za zhi = Chinese journal of contemporary pediatrics
(Zhongguo Dang Dai Er Ke Za Zhi)
Vol. 9
Issue 5
Pg. 473-8
(Oct 2007)
ISSN: 1008-8830 [Print] China |
PMID | 17937862
(Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)
|
Chemical References |
- RNA, Messenger
- Vascular Endothelial Growth Factor A
- Nitric Oxide
- Nitric Oxide Synthase Type III
|
Topics |
- Animals
- Bronchopulmonary Dysplasia
(etiology)
- Female
- Humans
- Hyperoxia
(metabolism)
- Infant, Newborn
- Lung
(metabolism, pathology)
- Male
- Nitric Oxide
(physiology)
- Nitric Oxide Synthase Type III
(genetics)
- RNA, Messenger
(analysis)
- Rats
- Rats, Sprague-Dawley
- Vascular Endothelial Growth Factor A
(genetics)
|