Toll-like receptor 2 (TLR) -196 to 174del polymorphism in gastro-duodenal diseases in Japanese population.

Abstract

Toll-like receptors (TLRs) play important roles in the signaling of many pathogen-related molecules and endogenous proteins associated with immune activation. -196 to -174del polymorphism affects the TLR2 gene and alters its promoter activity. We investigated the influence of TLR2 -196 to -174del polymorphism on the risk of gastro-duodenal diseases, on the severity of Helicobacter pylori-induced gastritis in a Japanese population. The study was performed on 309 patients with abdominal discomfort and 146 healthy controls. -196 to -174del polymorphism of TLR2 was investigated by allele-specific polymerase chain reaction method in all of the subjects. Gastritis scores of antral gastric mucosa were assessed according to the updated Sydney system in H. pylori-positive subjects (n = 156). Patients with abdominal discomfort was consisted of 80 gastric ulcers (25.9%), 38 duodenal ulcers (12.3%), five gastric + duodenal ulcers (1.6%), 105 patients with gastritis (34.0%) and 81 normal healthy stomachs (26.2%). We did not find any association between TLR2 polymorphism and risk of gastric ulcer, duodenal ulcer, gastric and duodenal ulcer and gastritis compared to healthy controls. However, the TLR2-196 to -174ins allele was associated with severity of intestinal metaplasia in more than 60 years of ages (P = 0.02). The same allele also increased the risks of developing more severe gastric mucosal atrophy and intestinal metaplasia in female subjects (P < 0.05, P = 0.07 respectively). No association was observed between TLR2 polymorphism and severity of neutrophil and mononuclear cell infiltration. Our data suggest that the TLR2-196 to -174ins allele was associated with more severe intestinal metaplasia in patients older than was correlated with severity of gastric mucosal atrophy and intestinal metaplasia in female subjects.

• Authors: Tomomitsu Tahara, Tomiyasu Arisawa, Fangyu Wang, Tomoyuki Shibata, Masakatsu Nakamura, Mikijyu Sakata, Ichiro Hirata, Hiroshi Nakano
• Journal: Digestive diseases and sciences (Dig Dis Sci) Vol. 53 Issue 4 Pg. 919-24 (Apr 2008) ISSN: 0163-2116 [Print] United States
• PMID: 17934843 (Publication Type: Journal Article)

Chemical References

• TLR2 protein, human
• Toll-Like Receptor 2

Topics

• Adult
• Aged
• Asian People (genetics)
• Case-Control Studies
• Cohort Studies
• Female
• Gastritis (ethnology, genetics, microbiology)
• Genotype
• Helicobacter Infections (complications, ethnology, genetics)
• Helicobacter pylori
• Humans
• Japan
• Male
• Middle Aged
• Peptic Ulcer (ethnology, genetics, microbiology)
• Polymorphism, Genetic
• Toll-Like Receptor 2 (genetics)