Evaluation of sphingolipids changes in brain tissues of rats with pentylenetetrazol-induced kindled seizures using MALDI-TOF-MS.

Abnormal lipid metabolism has been implicated in the pathogenesis of many neural system diseases, including epilepsy. Pentylenetetrazol (PTZ)-induced kindling in rodents is considered a model of human absence epilepsy and myoclonic, generalized tonic-clonic seizure. In an effort to further understand the mechanism for PTZ-induced seizure, we analyzed crude lipids and sphingolipids in the cortex, hippocampus, and brain stem of normal and PTZ-rats using delayed extraction matrix-assisted laser desorption ionization time-of-flight mass spectrometry (DE MALDI-TOF-MS). It was found that phosphatidylcholines dominated the crude lipids in different tissues and there were no obvious differences in crude lipid profiles of different tissues between normal and PTZ-rats. However, ceramide, sphingomyelins, and ceramide-monohexoside were differently distributed in normal and PTZ-rats. Using the reference mass spectra method established in our laboratory, it was shown that sphingomyelins and ceramide-monohexoside levels were elevated in the brain tissues of PTZ-rats. Ceramide levels were found to be higher in brain stem than in cortex and hippocampus of normal rats, and PTZ caused a general decrease in ceramide levels. These data suggest that changes in sphingolipid metabolism contribute to PTZ-induced seizure.
AuthorsXiaoqiong Ma, Guangyi Liu, Shuang Wang, Zhong Chen, Maode Lai, Ziyang Liu, Jun Yang
JournalJournal of chromatography. B, Analytical technologies in the biomedical and life sciences (J Chromatogr B Analyt Technol Biomed Life Sci) Vol. 859 Issue 2 Pg. 170-7 (Nov 15 2007) ISSN: 1570-0232 [Print] Netherlands
PMID17931985 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • Sphingolipids
  • Pentylenetetrazole
  • Animals
  • Brain (metabolism)
  • Brain Chemistry
  • Kindling, Neurologic (drug effects, metabolism)
  • Male
  • Pentylenetetrazole
  • Rats
  • Rats, Sprague-Dawley
  • Seizures (chemically induced, metabolism)
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
  • Sphingolipids (metabolism)

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